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Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88NF-κB Signaling Pathway
Joint Authors
Zhang, Xu
Fei, Xiaojun
Tao, Weiwei
Li, Jingbo
Shen, Hao
Wang, Xuanye
Liu, Hongquan
Xu, Yun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Modified Xijiao Dihuang (XJDH) decoction has been shown to exert powerful neuroprotective properties in clinical ischemic stroke treatment.
It consists of 4 Chinese herbs: Buffalo Horn, Paeonia suffruticosa Andrews, Rehmannia glutinosa (Gaertn.) DC, and Paeonia lactiflora Pall.
In the present study, the neuroprotective effect and specific mechanisms of XJDH in protecting PC12 cells from oxygen-glucose deprivation-induced injury were investigated.
It was found that OGD/R significantly decreased the cell viability and lactate dehydrogenase (LDH) activity and increased the release of IL-1β, IL-6, and TNF-α in PC12 cells, and these effects were suppressed by XJDH and one of its major active constituents, paeoniflorin.
Additionally, XJDH inhibited caspase-3 activity and reduced cleaved caspase-3 level.
Mechanistic studies showed that the expressions of TLR4, MyD88, TRAF6, and NF-κB p65 and phosphorylation of IκBα and p65 were significantly lower in the XJDH-treated group than in the OGD/R control group.
Additionally, XJDH reversed the OGD/R-induced increases in p-JNK and p-ERK1/2 expression.
These results suggest that XJDH protects PC12 cells from oxygen-glucose deprivation-induced injury, which may be associated with the inhibition of the TLR4-MyD88/NF-κB signaling pathway.
As an anti-inflammation factor, XJDH might be used as a neuronal protection strategy for the ischemia injury and related diseases.
American Psychological Association (APA)
Zhang, Xu& Fei, Xiaojun& Tao, Weiwei& Li, Jingbo& Shen, Hao& Wang, Xuanye…[et al.]. 2017. Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1153590
Modern Language Association (MLA)
Zhang, Xu…[et al.]. Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1153590
American Medical Association (AMA)
Zhang, Xu& Fei, Xiaojun& Tao, Weiwei& Li, Jingbo& Shen, Hao& Wang, Xuanye…[et al.]. Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88NF-κB Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1153590
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153590