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EWSR1 Rearrangement and CD99 Expression as Diagnostic Biomarkers for EwingPNET Sarcomas in a Moroccan Population
Joint Authors
Louati, Sara
Chbani, Laila
Bennis, Sanae
Senhaji, Nadia
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-18
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Ewing sarcoma/primitive neuroectodermal tumor (Ewing/PNET sarcomas or EPS) are a group of round cell tumors.
Malignant round cell tumors form a large and diverse group that includes rhabdomyosarcoma, synovial sarcoma, non-Hodgkin’s lymphoma, neuroblastoma, hepatoblastoma, Wilm’s tumor, desmoplastic small round cell tumor, and other morphologically similar entities.
Differential diagnosis of Ewing sarcoma/primitive neuroectodermal tumor (Ewing/PNET sarcomas or EPS) is difficult.
In addition to morphology and immunohistochemistry (IHC), differential diagnosis of these tumors is based on molecular analysis of the EWSR1 gene rearrangement using fluorescent in situ hybridization (FISH) technique.
We investigated the diagnostic value of combined CD99 immunostaining and EWSR1 t(22q12) alteration using a dual-color, break-apart rearrangement probe in forty-one formalin-fixed paraffin-embedded (FFPE) tissue samples from pediatric and adult patients diagnosed with EPS.
IHC was performed in all cases using the CD99 antibody and showed a positivity of 92.7% in the enrolled cases (38/41) followed by FISH analysis where 48.8% of the cases (20/41) were rearranged.
Sensitivity and specificity for IHC assays were 88% and 58%, respectively.
Notably, FISH had a sensitivity of 100% and a specificity of 87%.
In addition, CD99 positivity was found to correlate with EWSR1 rearrangement (p<0.05).
This report shows that FISH has better sensitivity and specificity than IHC in the Moroccan population, and supports its combination with CD99 immunostaining as diagnostic biomarkers for this rare malignant entity.”
American Psychological Association (APA)
Louati, Sara& Senhaji, Nadia& Chbani, Laila& Bennis, Sanae. 2018. EWSR1 Rearrangement and CD99 Expression as Diagnostic Biomarkers for EwingPNET Sarcomas in a Moroccan Population. Disease Markers،Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1153682
Modern Language Association (MLA)
Louati, Sara…[et al.]. EWSR1 Rearrangement and CD99 Expression as Diagnostic Biomarkers for EwingPNET Sarcomas in a Moroccan Population. Disease Markers No. 2018 (2018), pp.1-5.
https://search.emarefa.net/detail/BIM-1153682
American Medical Association (AMA)
Louati, Sara& Senhaji, Nadia& Chbani, Laila& Bennis, Sanae. EWSR1 Rearrangement and CD99 Expression as Diagnostic Biomarkers for EwingPNET Sarcomas in a Moroccan Population. Disease Markers. 2018. Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1153682
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153682