Metabolomics of Hydrazine-Induced Hepatotoxicity in Rats for Discovering Potential Biomarkers
Joint Authors
Lv, Yali
Liu, Lihong
An, Zhuoling
Li, Chao
Li, Pengfei
Wu, Cheng
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Metabolic pathway disturbances associated with drug-induced liver injury remain unsatisfactorily characterized.
Diagnostic biomarkers for hepatotoxicity have been used to minimize drug-induced liver injury and to increase the clinical safety.
A metabolomics strategy using rapid-resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS) analyses and multivariate statistics was implemented to identify potential biomarkers for hydrazine-induced hepatotoxicity.
The global serum and urine metabolomics of 30 hydrazine-treated rats at 24 or 48 h postdosing and 24 healthy rats were characterized by a metabolomics approach.
Multivariate statistical data analyses and receiver operating characteristic (ROC) curves were performed to identify the most significantly altered metabolites.
The 16 most significant potential biomarkers were identified to be closely related to hydrazine-induced liver injury.
The combination of these biomarkers had an area under the curve (AUC) > 0.85, with 100% specificity and sensitivity, respectively.
This high-quality classification group included amino acids and their derivatives, glutathione metabolites, vitamins, fatty acids, intermediates of pyrimidine metabolism, and lipids.
Additionally, metabolomics pathway analyses confirmed that phenylalanine, tyrosine, and tryptophan biosynthesis as well as tyrosine metabolism had great interactions with hydrazine-induced liver injury in rats.
These discriminating metabolites might be useful in understanding the pathogenesis mechanisms of liver injury and provide good prospects for drug-induced liver injury diagnosis clinically.
American Psychological Association (APA)
An, Zhuoling& Li, Chao& Lv, Yali& Li, Pengfei& Wu, Cheng& Liu, Lihong. 2018. Metabolomics of Hydrazine-Induced Hepatotoxicity in Rats for Discovering Potential Biomarkers. Disease Markers،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1153704
Modern Language Association (MLA)
An, Zhuoling…[et al.]. Metabolomics of Hydrazine-Induced Hepatotoxicity in Rats for Discovering Potential Biomarkers. Disease Markers No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1153704
American Medical Association (AMA)
An, Zhuoling& Li, Chao& Lv, Yali& Li, Pengfei& Wu, Cheng& Liu, Lihong. Metabolomics of Hydrazine-Induced Hepatotoxicity in Rats for Discovering Potential Biomarkers. Disease Markers. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1153704
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153704