Hypermethylation of APC2 Is a Predictive Epigenetic Biomarker for Chinese Colorectal Cancer
Joint Authors
He, Yuan
Sun, Li-Yue
Wang, Jing
Gong, Rui
Shao, Qiong
Zhang, Zi-Chen
Ye, Zu-Lu
Wang, Hai-Yun
Xu, Rui-Hua
Shao, Jian-Yong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
To investigate methylation of the adenomatosis polyposis coli homologue (APC2) promoter and its correlation with prognostic implications in Chinese colorectal cancer (CRC).
Methods.
The mRNA expression of APC2 in colorectal tissues was evaluated using the database of The Cancer Genome Atlas (TCGA).
Methylation analysis of APC2 in tumor (n=66) and corresponding adjacent formalin-fixed and paraffin-embedded (FFPE) tissues (n=44) was performed by Sequenom EpiTYPER® and verified by cloning-based bisulfite sequencing analysis.
Demethylation and retrieval of APC2 expression in cell lines HT29, HCT116, and SW480 were treated with 5-aza-2′-deoxycytidine (5-AZC).
Results.
Analysis of TCGA showed that APC2 mRNA was significantly downregulated in primary tumors when compared to normal tissues (p<0.05).
APC2 methylation was upregulated (43.93% vs 7.31%, p<0.05) in tumors compared to adjacent FFPE tissues.
In vitro experiments demonstrated that 5-AZC downregulated the methylation of APC2 and retrieved its expression of mRNA and protein levels (p<0.05).
Multivariate Cox regression indicated that APC2_CPG_14 was an independent risk factor for overall survival (HR = 6.38, 95% CI: 1.59–25.64, p<0.05).
Conclusion.
This study indicates that APC2 is hypermethylated and may be a tumorigenesis biomarker for Chinese CRC patients.
American Psychological Association (APA)
He, Yuan& Sun, Li-Yue& Wang, Jing& Gong, Rui& Shao, Qiong& Zhang, Zi-Chen…[et al.]. 2018. Hypermethylation of APC2 Is a Predictive Epigenetic Biomarker for Chinese Colorectal Cancer. Disease Markers،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1153712
Modern Language Association (MLA)
He, Yuan…[et al.]. Hypermethylation of APC2 Is a Predictive Epigenetic Biomarker for Chinese Colorectal Cancer. Disease Markers No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1153712
American Medical Association (AMA)
He, Yuan& Sun, Li-Yue& Wang, Jing& Gong, Rui& Shao, Qiong& Zhang, Zi-Chen…[et al.]. Hypermethylation of APC2 Is a Predictive Epigenetic Biomarker for Chinese Colorectal Cancer. Disease Markers. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1153712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153712