Effect of Acupotomy on FAK-PI3K Signaling Pathways in KOA Rabbit Articular Cartilages
Joint Authors
Guo, Chang Qing
Guo, Yan
Lu, Juan
Ma, Shi-Ning
Xie, Zhan-guo
Yu, Jia-Ni
Zhang, Wei
Wang, Li-Juan
Xu, Jing
Zhao, Rui-Li
Zhou, Shuai
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
By observing the needle-knife of KOA rabbit morphology, knee joint cartilage p-FAK, p-PI3K, Aggrecan gene, and protein expression, to study the effect of needle-knife to promote cartilage cell synthesis metabolism mechanism.
Method.
49 male New Zealand rabbits, randomly divided into normal group (Z), model group (M), model-inhibitors (MP), needle-knife group (D), needle-knife inhibitors group (DP), electroacupuncture group (E), and electroacupuncture inhibitors (EP).
RT-PCR and Western Blot were used to test each animal cartilage p-FAK, p-PI3K, and Aggrecan gene and protein expression level.
Results.
Compared with N group, p-FAK and p-PI3K protein and mRNA expression of M group, D group, and E group increased (P < 0.05), while the protein and mRNA expression of Aggrecan reduced (P < 0.05).
Compared with M group, p-FAK, p-PI3K, Aggrecan protein, and mRNA of E and D group increased (P < 0.05).
Compared with E group, p-FAK, p-PI3K, Aggrecan protein, and mRNA expression of D group increased (P < 0.05); after adding inhibitors, p-FAK, p-PI3K, Aggrecan protein, and mRNA expression reduced (P < 0.05).
Conclusion.
Needle-knife therapy can promote the repairment of cartilage cells by activating FAK-PI3K signaling pathways, promoting the synthesis of cartilage cell metabolism.
American Psychological Association (APA)
Ma, Shi-Ning& Xie, Zhan-guo& Guo, Yan& Yu, Jia-Ni& Lu, Juan& Zhang, Wei…[et al.]. 2017. Effect of Acupotomy on FAK-PI3K Signaling Pathways in KOA Rabbit Articular Cartilages. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1153772
Modern Language Association (MLA)
Ma, Shi-Ning…[et al.]. Effect of Acupotomy on FAK-PI3K Signaling Pathways in KOA Rabbit Articular Cartilages. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1153772
American Medical Association (AMA)
Ma, Shi-Ning& Xie, Zhan-guo& Guo, Yan& Yu, Jia-Ni& Lu, Juan& Zhang, Wei…[et al.]. Effect of Acupotomy on FAK-PI3K Signaling Pathways in KOA Rabbit Articular Cartilages. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1153772
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153772