Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma
Joint Authors
Zhang, Yingying
Liu, Xin
Liu, Liwen
Chen, Jianan
Hu, Qiuyue
Shen, Shen
Zhou, Yongjian
Chen, Siyu
Xue, Chen
Cui, Guangying
Yu, Zujiang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-13
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Background.
Studies show that patients with hepatocellular carcinoma (HCC) have poor prognosis, particularly when patients are diagnosed at late stages of the disease development.
The flap endonuclease 1 (FEN1) gene is overexpressed in multiple malignant tumors and may promote tumor aggressiveness.
However, its expression profile and functional roles in HCC are still unclear.
Here, we evaluated the molecular mechanisms of FEN1 in HCC.
Methods.
The expression of FEN1 in HCC was evaluated using HCC mRNA expression data from TCGA and GEO databases.
The expression of FEN1 was also confirmed by immunohistochemistry (IHC) using a tissue microarray (TMA) cohort with a total of 396 HCC patients.
Kaplan-Meier analysis and univariate and multivariate Cox regression analyses were used to determine the correlation between FEN1 expression and survival rate of HCC patients.
The molecular mechanism and biological functions of FEN1 in HCC were predicted using functional and pathway enrichment analysis in vitro experiments.
Results.
FEN1 was overexpressed in multiple HCC cohorts at both mRNA and protein levels.
The receiver operating characteristic (ROC) curve showed that FEN1 can serve as a diagnostic predictor of HCC.
Meanwhile, patients with high FEN1 expression levels showed lower overall survival (OS) and relapse-free survival (RFS) rates than those with low FEN1 expression.
More importantly, we found that FEN1 elevation was an independent prognostic factor for OS and RFS in HCC patients based on univariate and multivariate analyses, indicating that FEN1 might be a potential prognostic marker in HCC.
Furthermore, knocking down FEN1 resulted in suppressed cell proliferation and migration in vitro.
This could have been due to regulation expressions of c-Myc, survivin, and cyclin D1 genes, indicating that FEN1 may function as an oncogene through its role in the cell cycle and DNA replication pathway.
Conclusion.
Our study indicated that high FEN1 expression might function as a biomarker for diagnosis and prognosis.
In addition, the study confirms that FEN1 is an oncogene in HCC progression.
American Psychological Association (APA)
Zhang, Yingying& Liu, Xin& Liu, Liwen& Chen, Jianan& Hu, Qiuyue& Shen, Shen…[et al.]. 2020. Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma. Disease Markers،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1153796
Modern Language Association (MLA)
Zhang, Yingying…[et al.]. Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma. Disease Markers No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1153796
American Medical Association (AMA)
Zhang, Yingying& Liu, Xin& Liu, Liwen& Chen, Jianan& Hu, Qiuyue& Shen, Shen…[et al.]. Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1153796
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153796