Plasma-Derived Exosomal Circular RNA hsa_circ_0005540 as a Novel Diagnostic Biomarker for Coronary Artery Disease
Joint Authors
Liu, Xinguang
Chen, Can
Cai, Meng-yun
Cen, Jin-ming
Wu, Wei-peng
Pan, Yan-hong
Xiong, Xing-dong
Zheng, Lei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Exosomes exist in almost all body fluid and contain diverse biological contents which may be reflective of disease state.
Circular RNAs (circRNAs) are stable in structure and have a long half-life in exosomes without degradation, thus making them reliable biomarkers.
However, the potential of exosomal circRNAs as biomarkers of coronary artery disease (CAD) remains to be established.
Here, we aimed to investigate the expression levels and the potential use of exosomal circRNAs as diagnostic biomarkers for CAD.
Methods.
CircRNA expression levels in exosomes obtained from three plasma samples of CAD patients and three paired controls were analyzed using RNA sequencing.
Exosomal circRNAs obtained in the profiling phase were then verified in two-center validation cohorts.
Finally, the ability of exosomal circRNAs, adjusting for Framingham Heart Study (FHS) risk factors, was determined to discriminate between CAD patients and non-CAD controls.
Results.
355 circRNAs were differentially expressed between these two groups: 164 were upregulated, and 191 were downregulated.
Here, we selected the potential circRNAs (fold change>4, P<0.05) as candidate biomarkers for further validation.
Our data showed that only hsa_circ_0005540 was significantly associated with CAD (P<0.0001).
After adjustment for risk factors, hsa_circ_0005540 showed a high discriminatory power for CAD in ROC analyses (AUC=0.853; 95%confidence interval CI=0.799−0.906, P<0.001).
Conclusion.
Our results suggest that plasma exosomal hsa_circ_0005540 can be used as a promising diagnostic biomarker of CAD.
American Psychological Association (APA)
Wu, Wei-peng& Pan, Yan-hong& Cai, Meng-yun& Cen, Jin-ming& Chen, Can& Zheng, Lei…[et al.]. 2020. Plasma-Derived Exosomal Circular RNA hsa_circ_0005540 as a Novel Diagnostic Biomarker for Coronary Artery Disease. Disease Markers،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1153816
Modern Language Association (MLA)
Wu, Wei-peng…[et al.]. Plasma-Derived Exosomal Circular RNA hsa_circ_0005540 as a Novel Diagnostic Biomarker for Coronary Artery Disease. Disease Markers No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1153816
American Medical Association (AMA)
Wu, Wei-peng& Pan, Yan-hong& Cai, Meng-yun& Cen, Jin-ming& Chen, Can& Zheng, Lei…[et al.]. Plasma-Derived Exosomal Circular RNA hsa_circ_0005540 as a Novel Diagnostic Biomarker for Coronary Artery Disease. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1153816
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153816