ZNF143 Suppresses Cell Apoptosis and Promotes Proliferation in Gastric Cancer via ROSp53 Axis
Joint Authors
Zhang, Yi
Li, Qing
Wei, Song
Sun, Jing
Zhang, Xuan
He, Ling
Zhang, Lu
Xu, Zekuan
Chen, Dexuan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-04
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Aim.
This study was aimed at identifying the role of zinc finger protein 143 (ZNF143) in gastric cancer (GC) progression.
Methods.
The impact of ZNF143 on the proliferation ability and apoptosis of GC cells was detected.
The expression of ZNF143 and related targeted genes was determined using Western blot analysis.
The reactive oxygen species (ROS) level of GC cells was examined using the ROS generation assay.
The role of ZNF143 in the proliferation of GC cells in vivo was examined using tumor xenograft assay.
Results.
The ectopic overexpression of ZNF143 promoted the proliferation of GC cells, while its knockdown reduced the effect in vitro.
The downregulation of ZNF143 facilitated cell apoptosis.
ZNF143 decreased the ROS level in GC cells, resulting in the reduction of cell apoptosis.
Transfection with p53 reversed the antiapoptotic effect of ZNF143, while pifithrin-α, a specific inhibitor of p53, reduced the apoptosis in ZNF143-knockdown GC cells.
However, p53 had no influence on the ROS level in GC cells.
p53 played a key role in inhibiting ROS generation in GC cells, thereby inhibiting apoptosis.
The transplanted tumor weight and volume were higher in the ZNF143-overexpressed group than in the ZNF143-knockdown group in vivo.
The expression of ZNF143 in the transplanted tumor correlated positively with the proliferation index of Ki67 and negatively with the expression of p53 and cell apoptosis.
Conclusion.
ZNF143, as a tumor oncogene, promoted the proliferation of GC cells both in vitro and in vivo, indicating that ZNF143 might function as a novel target for GC therapy.
American Psychological Association (APA)
Zhang, Yi& Li, Qing& Wei, Song& Sun, Jing& Zhang, Xuan& He, Ling…[et al.]. 2020. ZNF143 Suppresses Cell Apoptosis and Promotes Proliferation in Gastric Cancer via ROSp53 Axis. Disease Markers،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1153902
Modern Language Association (MLA)
Zhang, Yi…[et al.]. ZNF143 Suppresses Cell Apoptosis and Promotes Proliferation in Gastric Cancer via ROSp53 Axis. Disease Markers No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1153902
American Medical Association (AMA)
Zhang, Yi& Li, Qing& Wei, Song& Sun, Jing& Zhang, Xuan& He, Ling…[et al.]. ZNF143 Suppresses Cell Apoptosis and Promotes Proliferation in Gastric Cancer via ROSp53 Axis. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1153902
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153902