Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-CEBP-βc-Fos-MMP-7 Pathways
Joint Authors
Liu, Hsiang-Chun
Tzeng, Yew-Min
Chen, Tzu-Hsiu
Chiu, Kun-Yuan
Wen, Chi-Luan
Lai, Jin-Mei
Cheng, Chi-Chih
Hsu, Shih-Lan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-02
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Antcin-H, a natural triterpene, is purified from a famous anticancer medicinal mushroom, Antrodia cinnamomea, in Taiwan.
This study showed that antcin-H inhibited the growth of human renal carcinoma 786-0 cells; the IC50 value (for 48 h) was 170 μM.
Besides, the migration and invasion of 786-0 cells were suppressed by antcin-H under noncytotoxic concentrations (<100 μM); these events were accompanied by inhibition of FAK and Src kinase activities, decrease of paxillin phosphorylation, impairment of lamellipodium formation, and upregulation of TIMPs and downregulation of MMPs, especially MMP-7 expression.
Luciferase reporter assay showed that antcin-H repressed the MMP-7 promoter activity, in parallel to inhibiting c-Fos/AP-1 and C/EBP-β transactivation abilities.
Moreover, antcin-H suppressed the activity of ERK1/2 and decreased the binding ability of C/EBP-β and c-Fos on the upstream/enhancer region of MMP-7 promoter.
Overall, this study demonstrated that the anti-invasive effect of antcin-H in human renal carcinoma 786-0 cells might be at least in part by abrogating focal adhesion complex and lamellipodium formation through inhibiting the Src/FAK-paxillin signaling pathways and decreasing MMP-7 expression through suppressing the ERK1/2-AP-1/c-Fos and C/EBP-β signaling axis.
Our findings provide the evidence that antcin-H may be an active component existing in A.
cinnamomea with anticancer effect.
American Psychological Association (APA)
Chiu, Kun-Yuan& Chen, Tzu-Hsiu& Wen, Chi-Luan& Lai, Jin-Mei& Cheng, Chi-Chih& Liu, Hsiang-Chun…[et al.]. 2017. Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-CEBP-βc-Fos-MMP-7 Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1153945
Modern Language Association (MLA)
Chiu, Kun-Yuan…[et al.]. Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-CEBP-βc-Fos-MMP-7 Pathways. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1153945
American Medical Association (AMA)
Chiu, Kun-Yuan& Chen, Tzu-Hsiu& Wen, Chi-Luan& Lai, Jin-Mei& Cheng, Chi-Chih& Liu, Hsiang-Chun…[et al.]. Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-CEBP-βc-Fos-MMP-7 Pathways. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1153945
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153945