Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Metastasis via the miR-155-5pFGF7 Axis and Predicts Poor Prognosis in Gastric Cancer
Joint Authors
Ma, Hong-Wu
Xi, Da-Yong
Ma, Jian-Zhong
Guo, Min
Ma, Li
Ma, Dong-Hong
Li, Pei-Wu
Guo, Chang-An
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Actin filament-associated protein 1-antisense RNA 1 (AFAP1-AS1) plays an important role in the development and progression of several human cancers.
However, its biological function in gastric cancer (GC) progression is still unknown.
Methods.
We used qRT-PCR to detect the relative expression of AFAP1-AS1 in GC tissues and cell lines.
The loss-of-function assays were conducted to detect the effect of AFAP1-AS1 on GC development.
Bioinformatics analysis, luciferase reporter gene analysis, and RIP analysis were used to identify and validate target genes of AFAP1-AS1.
Finally, rescue tests were performed to confirm the influence of the AFAP1-AS1-miR-155-5p-FGF7 axis on GC development.
Results.
AFAP1-AS1 was upregulated in GC tissues and cell lines and was closely correlated with poor prognosis of GC patients.
AFAP1-AS1 knockdown inhibited proliferation, migration, and invasion of GC cells, indicating that AFAP1-AS1 acts as an oncogene in GC.
Bioinformatics analysis, dual-luciferase reporter gene detection, and RIP assays validated that AFAP1-AS1 directly interacts to miR-155-5p and could positively affect cell proliferation, migration, and invasion by regulation of the expression of miR-155-5p and FGF7.
Further rescue assays revealed that AFAP1-AS1 promotes cell proliferation and metastasis through the miR-155-5p/FGF7 axis in GC.
Conclusions.
AFAP1-AS1 might be an oncogenic lncRNA that promoted GC progression by acting as a competing endogenous RNA (ceRNA) that regulates the expression of FGF7 through sponging miR-155-5p, suggesting that AFAP1-AS1 may be a novel potential therapeutic target for GC.
American Psychological Association (APA)
Ma, Hong-Wu& Xi, Da-Yong& Ma, Jian-Zhong& Guo, Min& Ma, Li& Ma, Dong-Hong…[et al.]. 2020. Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Metastasis via the miR-155-5pFGF7 Axis and Predicts Poor Prognosis in Gastric Cancer. Disease Markers،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1154018
Modern Language Association (MLA)
Ma, Hong-Wu…[et al.]. Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Metastasis via the miR-155-5pFGF7 Axis and Predicts Poor Prognosis in Gastric Cancer. Disease Markers No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1154018
American Medical Association (AMA)
Ma, Hong-Wu& Xi, Da-Yong& Ma, Jian-Zhong& Guo, Min& Ma, Li& Ma, Dong-Hong…[et al.]. Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Metastasis via the miR-155-5pFGF7 Axis and Predicts Poor Prognosis in Gastric Cancer. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1154018
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154018