Acyclic Sesquiterpenes from the Fruit Pericarp of Sapindus saponaria Induce Ultrastructural Alterations and Cell Death in Leishmania amazonensis
Joint Authors
Nakamura, Celso Vataru
Moreira, Amanda Louzano
Scariot, Débora Botura
Pelegrini, Bruna Luíza
Pessini, Greisiele Lorena
Ueda-Nakamura, Tânia
Ferreira, Izabel Cristina Piloto
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Previous studies reported antiprotozoal activities of Sapindus saponaria L.
The aim of this work was the evaluation of antileishmanial activity and mechanism of action of extract and fractions of S.
saponaria L.
Hydroethanolic extract (EHA) obtained from fruit pericarps was fractionated using solid-phase extraction in a reversed phase, resulting in fractions enriched with saponins (SAP fraction) and acyclic sesquiterpene oligoglycosides (OGSA fraction).
The activities of EHA, SAP, and OGSA were evaluated by antiproliferative assays with promastigote and intracellular amastigote forms.
Cytotoxicity on macrophages and hemolytic activity were also analyzed.
Morphological and ultrastructural changes in Leishmania amazonensis promastigotes were evaluated by electron microscopy.
Flow cytometry was used to investigate mitochondrial dysfunction and phosphatidylserine exposure.
OGSA was more selective for parasites than mammalian J774A1 macrophage cells, with selectivity indices of 3.79 and 7.35, respectively.
Our results showed that only the OGSA fraction did not present hemolytic activity at its IC50 for promastigote growth.
Electron microscopy revealed changes in parasite flagellum, cell body shape, and organelle size, mainly mitochondria.
Flow cytometry analysis indicated mitochondrial membrane and cell membrane dysfunction.
OGSA showed antileishmanial activity, resulting in several changes to protozoa cells, including mitochondrial depolarization and early phosphatidylserine exposure, suggesting a possible apoptotic induction.
American Psychological Association (APA)
Moreira, Amanda Louzano& Scariot, Débora Botura& Pelegrini, Bruna Luíza& Pessini, Greisiele Lorena& Ueda-Nakamura, Tânia& Nakamura, Celso Vataru…[et al.]. 2017. Acyclic Sesquiterpenes from the Fruit Pericarp of Sapindus saponaria Induce Ultrastructural Alterations and Cell Death in Leishmania amazonensis. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1154078
Modern Language Association (MLA)
Moreira, Amanda Louzano…[et al.]. Acyclic Sesquiterpenes from the Fruit Pericarp of Sapindus saponaria Induce Ultrastructural Alterations and Cell Death in Leishmania amazonensis. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1154078
American Medical Association (AMA)
Moreira, Amanda Louzano& Scariot, Débora Botura& Pelegrini, Bruna Luíza& Pessini, Greisiele Lorena& Ueda-Nakamura, Tânia& Nakamura, Celso Vataru…[et al.]. Acyclic Sesquiterpenes from the Fruit Pericarp of Sapindus saponaria Induce Ultrastructural Alterations and Cell Death in Leishmania amazonensis. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1154078
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154078