Associations between Interleukin-32 Gene Polymorphisms rs12934561 and rs28372698 and Susceptibilities to Bladder Cancer and the Prognosis in Chinese Han Population

Abstract EN

The proinflammatory chemokine interleukin-32 is related to various diseases, including cancer.

However, it has never been associated with bladder cancer (BC).

To detect whether there is a relationship between the IL-32 gene polymorphisms (rs12934561 C/T and rs28372698 T/A) and BC, the study enrolled 170 non-muscle-invasive bladder cancer (NMIBC) patients, 151 muscle-invasive bladder cancer (MIBC) patients, and 437 healthy controls.

The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for the IL-32 single-nucleotide polymorphism (SNP) genotyping.

Statistical analysis was performed using SNPstats online analysis software and SPSS software.

Our data revealed that the CC homozygous genotype of rs12934561 in BC patients was significantly higher than that in controls (P=0.03, OR=1.47, 95%CI=1.04‐2.08), and the percentage of TC genotype carriers was relatively less than that of controls (P=0.001, OR=0.61, 95%CI=0.45‐0.82).

Furthermore, the TT homozygous genotype of rs28372698 was associated with a significantly lower overall survival rate in MIBC patients (P=0.028, OR=2.77, 95%CI=1.11‐6.90).

The IL-32 gene polymorphism rs12934561 might be associated with increased BC risk, and the rs28372698 might participate in the prognosis of BC patients.

Therefore, they could be potential forecasting factors for the prognosis of MIBC patients.