Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes
Joint Authors
Chiranthanut, Natthakarn
Panthong, Ampai
Khonsung, Parirat
Na Takuathung, Mingkwan
Wongnoppavich, Ariyaphong
Pitchakarn, Pornsiri
Soonthornchareonnon, Noppamas
Sireeratawong, Seewaboon
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Psoriasis is a chronic inflammatory and immune-mediated skin disease.
The pathogenesis involves T cells activation via the IL-23/Th17 axis.
Conventional treatments of psoriasis have adverse events influencing patients’ adherence.
Wannachawee Recipe (WCR) has been effectively used as Thai folk remedy for psoriasis patients; however, preclinical evidence defining how WCR works is still lacking.
This study defined mechanisms for its antiproliferation and anti-inflammatory effects in HaCaT cells.
The cytotoxicity and antiproliferation results from SRB and CCK-8 assays showed that WCR inhibited the growth and viability of HaCaT cells in a concentration-dependent manner.
The distribution of cell cycle phases determined by flow cytometry showed that WCR did not interrupt cell cycle progression.
Interestingly, RT-qPCR revealed that WCR significantly decreased the mRNA expression of IL-1β, IL-6, IL-8, IL-17A, IL-22, IL-23, and TNF-α but induced IL-10 expression in TNF-α- and IFN-γ-induced HaCaT cells.
At the protein level determined by ELISA, WCR significantly reduced the secretion of IL-17A, IL-22, and IL-23.
The WCR at low concentrations was proved to possess anti-inflammatory effect without cytotoxicity and it did not interfere with cell cycle of keratinocytes.
This is the first study to provide convincing evidence that WCR is a potential candidate for development of effective psoriasis therapies.
American Psychological Association (APA)
Na Takuathung, Mingkwan& Wongnoppavich, Ariyaphong& Pitchakarn, Pornsiri& Panthong, Ampai& Khonsung, Parirat& Chiranthanut, Natthakarn…[et al.]. 2017. Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1154151
Modern Language Association (MLA)
Na Takuathung, Mingkwan…[et al.]. Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1154151
American Medical Association (AMA)
Na Takuathung, Mingkwan& Wongnoppavich, Ariyaphong& Pitchakarn, Pornsiri& Panthong, Ampai& Khonsung, Parirat& Chiranthanut, Natthakarn…[et al.]. Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1154151
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154151