Cryptotanshinone Regulates Androgen Synthesis through the ERKc-FosCYP17 Pathway in Porcine Granulosa Cells
Joint Authors
Wu, Xiaoke
Ng, Ernest Hung Yu
Zhang, Yue-Hui
Ye, Danfeng
Li, Meifang
Wang, Xinhua
Liu, Hua
Wu, Wanting
Ma, Wanying
Quan, Kewei
Lai, Maohua
Ma, Hongxia
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of the study is to investigate the molecular mechanism behind androgen reduction in porcine granulosa cells (pGCs) with Salvia miltiorrhiza Bunge extract cryptotanshinone.
PGCs were isolated from porcine ovaries and identified.
Androgen excess model of the pGCs was induced with the MAPK inhibitor PD98059 and then treated with cryptotanshinone.
The testosterone level was measured by radioimmunoassay in the culture media.
The protein levels of P-ERK1/2, c-Fos, and CYP17 in the cells were measured by western blot.
Cryptotanshinone decreased the concentration of testosterone and the protein level of CYP17 and increased the protein levels of P-ERK1/2 and c-Fos in the androgen excess mode.
After the c-Fos gene was silenced by infection with c-Fos shRNA lentivirus, we measured the mRNA expression by quantitative RT-PCR and protein level by western blot of P-ERK1/2, c-Fos, and CYP17.
This showed that the mRNA expression and protein level of P-ERK1/2 and c-Fos were significantly reduced in the shRNA–c-Fos group compared to the scrambled group, while those of CYP17 were significantly increased.
So we concluded that cryptotanshinone can significantly reduce the androgen excess induced by PD98059 in pGCs.
The possible molecular mechanism for this activity is regulating the ERK/c-Fos/CYP17 pathway.
American Psychological Association (APA)
Ye, Danfeng& Li, Meifang& Zhang, Yue-Hui& Wang, Xinhua& Liu, Hua& Wu, Wanting…[et al.]. 2017. Cryptotanshinone Regulates Androgen Synthesis through the ERKc-FosCYP17 Pathway in Porcine Granulosa Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154169
Modern Language Association (MLA)
Ye, Danfeng…[et al.]. Cryptotanshinone Regulates Androgen Synthesis through the ERKc-FosCYP17 Pathway in Porcine Granulosa Cells. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154169
American Medical Association (AMA)
Ye, Danfeng& Li, Meifang& Zhang, Yue-Hui& Wang, Xinhua& Liu, Hua& Wu, Wanting…[et al.]. Cryptotanshinone Regulates Androgen Synthesis through the ERKc-FosCYP17 Pathway in Porcine Granulosa Cells. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154169
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154169