Detection of BRAF V600E Mutation in Ganglioglioma and Pilocytic Astrocytoma by Immunohistochemistry and Real-Time PCR-Based Idylla Test
Joint Authors
Szylberg, Łukasz
Grzanka, Dariusz
Antosik, Paulina
Kasperska, Anna
Durślewicz, Justyna
Klimaszewska-Wiśniewska, Anna
Szylberg, Tadeusz
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The BRAF V600E mutation is an important oncological target in certain central nervous system (CNS) tumors, for which a possible application of BRAF-targeted therapy grows continuously.
In the present study, we aim to determine the prevalence of BRAF V600E mutations in a series of ganglioglioma (GG) and pilocytic astrocytoma (PA) cases.
Simultaneously, we decided to verify whether the combination of fully automated tests—BRAF-VE1 immunohistochemistry (IHC) and Idylla BRAF mutation assay—may be useful to accurately predict it in the case of specified CNS tumors.
The study included 49 formalin-fixed, paraffin-embedded tissues, of which 15 were GG and 34 PA.
Immunohistochemistry with anti-BRAF V600E (VE1) antibody was performed on tissue sections using the VentanaBenchMark ULTRA platform.
All positive or equivocal cases on IHC and selected negative ones were further assessed using the Idylla BRAF mutation assay coupled with the Idylla platform.
The BRAF-VE1 IHC was positive in 6 (6/49; 12.3%) and negative in 39 samples (39/49; 79.6%).
The interpretation of immunostaining results was complicated in 4 cases, of which 1 tested positive for the Idylla BRAF mutation assay.
Therefore, the overall positivity rate was 14.3%.
This included 2 cases of GG and 5 cases of PA.
Our study found that BRAF V600E mutations are moderately frequent in PA and GG and that for these tumor entities, IHC VE1 is suitable for screening purposes, but all negative, equivocal, and weak positive cases should be further tested with molecular biology techniques, of which the Idylla system seems to be a promising tool.
American Psychological Association (APA)
Durślewicz, Justyna& Klimaszewska-Wiśniewska, Anna& Antosik, Paulina& Kasperska, Anna& Grzanka, Dariusz& Szylberg, Tadeusz…[et al.]. 2020. Detection of BRAF V600E Mutation in Ganglioglioma and Pilocytic Astrocytoma by Immunohistochemistry and Real-Time PCR-Based Idylla Test. Disease Markers،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1154171
Modern Language Association (MLA)
Durślewicz, Justyna…[et al.]. Detection of BRAF V600E Mutation in Ganglioglioma and Pilocytic Astrocytoma by Immunohistochemistry and Real-Time PCR-Based Idylla Test. Disease Markers No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1154171
American Medical Association (AMA)
Durślewicz, Justyna& Klimaszewska-Wiśniewska, Anna& Antosik, Paulina& Kasperska, Anna& Grzanka, Dariusz& Szylberg, Tadeusz…[et al.]. Detection of BRAF V600E Mutation in Ganglioglioma and Pilocytic Astrocytoma by Immunohistochemistry and Real-Time PCR-Based Idylla Test. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1154171
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154171