Vitamin E-Mediated Modulation of Glutamate Receptor Expression in an Oxidative Stress Model of Neural Cells Derived from Embryonic Stem Cell Cultures
Joint Authors
Nordin, Norshariza
Abd Jalil, Afifah
Khaza’ai, Huzwah
Mansor, Nur’izzati
Zaulkffali, Amirah Salwani
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Glutamate is the primary excitatory neurotransmitter in the central nervous system.
Excessive concentrations of glutamate in the brain can be excitotoxic and cause oxidative stress, which is associated with Alzheimer’s disease.
In the present study, the effects of vitamin E in the form of tocotrienol-rich fraction (TRF) and alpha-tocopherol (α-TCP) in modulating the glutamate receptor and neuron injury markers in an in vitro model of oxidative stress in neural-derived embryonic stem (ES) cell cultures were elucidated.
A transgenic mouse ES cell line (46C) was differentiated into a neural lineage in vitro via induction with retinoic acid.
These cells were then subjected to oxidative stress with a significantly high concentration of glutamate.
Measurement of reactive oxygen species (ROS) was performed after inducing glutamate excitotoxicity, and recovery from this toxicity in response to vitamin E was determined.
The gene expression levels of glutamate receptors and neuron-specific enolase were elucidated using real-time PCR.
The results reveal that neural cells derived from 46C cells and subjected to oxidative stress exhibit downregulation of NMDA, kainate receptor, and NSE after posttreatment with different concentrations of TRF and α-TCP, a sign of neurorecovery.
Treatment of either TRF or α-TCP reduced the levels of ROS in neural cells subjected to glutamate-induced oxidative stress; these results indicated that vitamin E is a potent antioxidant.
American Psychological Association (APA)
Abd Jalil, Afifah& Khaza’ai, Huzwah& Nordin, Norshariza& Mansor, Nur’izzati& Zaulkffali, Amirah Salwani. 2017. Vitamin E-Mediated Modulation of Glutamate Receptor Expression in an Oxidative Stress Model of Neural Cells Derived from Embryonic Stem Cell Cultures. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1154180
Modern Language Association (MLA)
Abd Jalil, Afifah…[et al.]. Vitamin E-Mediated Modulation of Glutamate Receptor Expression in an Oxidative Stress Model of Neural Cells Derived from Embryonic Stem Cell Cultures. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1154180
American Medical Association (AMA)
Abd Jalil, Afifah& Khaza’ai, Huzwah& Nordin, Norshariza& Mansor, Nur’izzati& Zaulkffali, Amirah Salwani. Vitamin E-Mediated Modulation of Glutamate Receptor Expression in an Oxidative Stress Model of Neural Cells Derived from Embryonic Stem Cell Cultures. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1154180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154180