Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model
Joint Authors
Sakaue, S.
Asano, Kazuhito
Sunagawa, Masataka
Ishikawa, Takako
Tezuka, Chiaki
Hisamitsu, T.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Introduction.
Medical care for Japanese cancer patients includes Western and Kampo medicines, and treatments with juzentaihoto (JTT) reportedly prevent cancer metastasis and recurrence.
In this study, we examined the effects of JTT on natural killer (NK) cell activity and metastasis in combined treatments with anti-PD-1 antibody in a mouse model of melanoma metastasis.
Methods.
C57BL/6 male mice were intravenously injected with B16 melanoma cells (B16 cell) and were given chow containing 3% JTT.
In subsequent in vivo experiments, we assessed serum cytokine levels and tumor colony formation in the lungs.
Additionally, we assessed NK cell activity in ex vivo experiments.
Results.
JTT significantly suppressed B16 cell metastasis, whereas injection of anti-asialo-GM1 antibody into mice abrogated the inhibitory actions of JTT.
JTT significantly increased interleukin- (IL-) 12 and interferon- (IFN-) γ levels in serum and induced NK cell activity.
It increased the inhibitory actions of the anti-PD-1 antibody on B16 cell metastasis.
Discussion.
These data suggest that JTT inhibits B16 cell metastasis by inducing NK cell activity.
Additionally, combination therapy with JTT and anti-PD-1 antibody increased treatment response rates for B16 melanoma.
American Psychological Association (APA)
Sakaue, S.& Ishikawa, Takako& Tezuka, Chiaki& Asano, Kazuhito& Sunagawa, Masataka& Hisamitsu, T.. 2017. Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1154185
Modern Language Association (MLA)
Sakaue, S.…[et al.]. Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1154185
American Medical Association (AMA)
Sakaue, S.& Ishikawa, Takako& Tezuka, Chiaki& Asano, Kazuhito& Sunagawa, Masataka& Hisamitsu, T.. Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1154185
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154185