The Evaluation of Toxicity Induced by Psoraleae Fructus in Rats Using Untargeted Metabonomic Method Based on UPLC-Q-TOFMS
Joint Authors
Li, Yu-bo
Zhang, Yanjun
Xu, Yanyan
Zhao, Yiwei
Xie, Jiabin
Sheng, Xue
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Psoraleae Fructus is the dry and mature fruit of leguminous plant Psoralea corylifolia L., with the activity of warming kidney and enhancing yang, warming spleen, and other effects.
However, large doses can cause liver and kidney toxicity.
Therefore, it is necessary to evaluate the toxicity of Psoraleae Fructus systematically.
Although traditional biochemical indicators and pathological tests have been used to evaluate the safety of drug, these methods lack sensitivity and specificity, so a fast and sensitive analytical method is urgently needed.
In this study, an ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) method was used to analyze the metabolic profiles of rat plasma.
The changes of metabolites in plasma samples were detected by partial least squares-discriminant analysis (PLS-DA).
Compared with the control group, after 7 days of administration, the pathological sections showed liver and kidney toxicity, and the metabolic trend was changed.
Finally, 13 potential biomarkers related to the toxicity of Psoraleae Fructus were screened.
The metabolic pathways involved were glycerol phospholipids metabolism, amino acid metabolism, energy metabolism, and so forth.
The discovery of these biomarkers laid a foundation for better explaining the hepatotoxicity and nephrotoxicity of Psoraleae Fructus and provided a guarantee for its safety evaluation.
American Psychological Association (APA)
Xu, Yanyan& Zhao, Yiwei& Xie, Jiabin& Sheng, Xue& Li, Yu-bo& Zhang, Yanjun. 2017. The Evaluation of Toxicity Induced by Psoraleae Fructus in Rats Using Untargeted Metabonomic Method Based on UPLC-Q-TOFMS. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154225
Modern Language Association (MLA)
Xu, Yanyan…[et al.]. The Evaluation of Toxicity Induced by Psoraleae Fructus in Rats Using Untargeted Metabonomic Method Based on UPLC-Q-TOFMS. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1154225
American Medical Association (AMA)
Xu, Yanyan& Zhao, Yiwei& Xie, Jiabin& Sheng, Xue& Li, Yu-bo& Zhang, Yanjun. The Evaluation of Toxicity Induced by Psoraleae Fructus in Rats Using Untargeted Metabonomic Method Based on UPLC-Q-TOFMS. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154225
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154225