Association Study of Coronary Artery Disease-Associated Genome-Wide Significant SNPs with Coronary Stenosis in Pakistani Population

Abstract EN

Genome-wide association studies (GWAS) of coronary artery disease (CAD) have revealed multiple genetic risk loci.

We assessed the association of 47 genome-wide significant single-nucleotide polymorphisms (SNPs) at 43 CAD loci with coronary stenosis in a Pakistani sample comprising 663 clinically ascertained and angiographically confirmed cases.

Genotypes were determined using the iPLEX Gold technology.

All statistical analyses were performed using R software.

Linkage disequilibrium (LD) between significant SNPs was determined using SNAP web portal, and functional annotation of SNPs was performed using the RegulomeDB and Genotype-Tissue Expression (GTEx) databases.

Genotyping comparison was made between cases with severe stenosis (≥70%) and mild/minimal stenosis (<30%).

Five SNPs demonstrated significant associations: three with additive genetic models PLG/rs4252120 (p=0.0078), KIAA1462/rs2505083 (p=0.005), and SLC22A3/rs2048327 (p=0.045) and two with recessive models SORT1/rs602633 (p=0.005) and UBE2Z/rs46522 (p=0.03).

PLG/rs4252120 was in LD with two functional PLG variants (rs4252126 and rs4252135), each with a RegulomeDB score of 1f.

Likewise, KIAA1462/rs2505083 was in LD with a functional SNP, KIAA1462/rs3739998, having a RegulomeDB score of 2b.

In the GTEx database, KIAA1462/rs2505083, SLC22A3/rs2048327, SORT1/rs602633, and UBE2Z/rs46522 SNPs were found to be expression quantitative trait loci (eQTLs) in CAD-associated tissues.

In conclusion, five genome-wide significant SNPs previously reported in European GWAS were replicated in the Pakistani sample.

Further association studies on larger non-European populations are needed to understand the worldwide genetic architecture of CAD.