Dahuang Zhechong Pill Combined with Doxorubicin Induces Cell Death through Regulating Energy Metabolism in Human Hepatocellular Carcinoma Cells
Joint Authors
Chen, Zhipeng
Li, Weidong
Liu, Xiao
Wu, Li
Zhao, Jiayu
Wang, Jiaqi
Cai, Hao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Many physiological activities such as cell survival, proliferation, defense, adaptation, and metabolism need to consume energy.
Hepatoma cells can quickly start stress responses like multidrug resistance (MDR) requiring adenosine triphosphate (ATP) consumption after administration of chemotherapeutics.
We employed CCK-8 assay to evaluate cell viability and the flow cytometry to confirm apoptosis and necrosis.
ELISA kit was used to determine intracellular levels of ATP in lysates.
Western blot was employed to analyze the expressions of key enzymes involved in energy metabolism.
We found that doxorubicin (DOX) potently stimulated apoptosis at a low dose and even induced necrosis at a high dose in SMMC-7721.
DHZCP combined with DOX at low or middle dose enhanced the synergistic antihepatoma effect.
Results indicated that Dahuang Zhechong Pill (DHZCP) inhibited the expressions of several key enzymes involved in oxidative phosphorylation and reduced intracellular ATP levels.
The combination of DHZCP with DOX reversed the elevation of intracellular ATP levels, and a significantly synergistic antitumor effect was observed.
DHZCP could not only strengthen the therapeutic effects of chemotherapeutic drugs but also decrease the doses of chemotherapeutic drugs and the incidences of adverse reactions, providing novel strategies for clinical treatment of liver cancer.
American Psychological Association (APA)
Wu, Li& Zhao, Jiayu& Cai, Hao& Wang, Jiaqi& Chen, Zhipeng& Li, Weidong…[et al.]. 2017. Dahuang Zhechong Pill Combined with Doxorubicin Induces Cell Death through Regulating Energy Metabolism in Human Hepatocellular Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1154253
Modern Language Association (MLA)
Wu, Li…[et al.]. Dahuang Zhechong Pill Combined with Doxorubicin Induces Cell Death through Regulating Energy Metabolism in Human Hepatocellular Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1154253
American Medical Association (AMA)
Wu, Li& Zhao, Jiayu& Cai, Hao& Wang, Jiaqi& Chen, Zhipeng& Li, Weidong…[et al.]. Dahuang Zhechong Pill Combined with Doxorubicin Induces Cell Death through Regulating Energy Metabolism in Human Hepatocellular Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1154253
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154253