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Effects of Angelica Extract on Schwann Cell Proliferation and Expressions of Related Proteins
Joint Authors
Jiang, Xiaowen
Feng, Xinxin
Qiao, Lu
Liu, Lin
Zhang, Binqing
Yu, Wenhui
Lu, Ziyin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The present study investigated the effects of Angelica extract (AE) on Schwann cell proliferation and expressions of related proteins, including brain derived neurotrophic factor (BDNF), neural cell adhesion molecule (NCAM), and proliferating cell nuclear antigen (PCNA).
Proliferation activity and cell cycles of SCs were evaluated by MTT assay and flow cytometry methods, respectively, after 12 h treatment of AE at different concentrations (62.5, 125, 250, 1000, 2000, 4000, and 8000 mg/L).
SCs were treated by 500, 1000, and 2000 mg/L AE for 24 h or 48 h; the related genes mRNA and proteins expressions in SCs were detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) kit.
At the concentration range of 125–2000 mg/L, the SC proliferation was induced by AE in a dose-dependent manner, especially 1000 and 2000 mg/L; cells in drug-treated groups showed the most increase.
Cells counts were ascended significantly in (G2/M + S) phase compared to control group.
BDNF, NCAM, and PCNA protein expressions significantly increased at drug-treated groups.
Relative genes mRNA expressions levels were also significantly higher compared to control group.
The results indicated that AE facilitated SC proliferation and related genes and proteins expressions, which provided a basic guideline for nerve injury repair in clinic.
American Psychological Association (APA)
Jiang, Xiaowen& Liu, Lin& Zhang, Binqing& Lu, Ziyin& Qiao, Lu& Feng, Xinxin…[et al.]. 2017. Effects of Angelica Extract on Schwann Cell Proliferation and Expressions of Related Proteins. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154267
Modern Language Association (MLA)
Jiang, Xiaowen…[et al.]. Effects of Angelica Extract on Schwann Cell Proliferation and Expressions of Related Proteins. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1154267
American Medical Association (AMA)
Jiang, Xiaowen& Liu, Lin& Zhang, Binqing& Lu, Ziyin& Qiao, Lu& Feng, Xinxin…[et al.]. Effects of Angelica Extract on Schwann Cell Proliferation and Expressions of Related Proteins. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154267
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154267