Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro
Joint Authors
Reheman, Ayinuer
Ma, Qing Ling
Nijat, Dilaram
Abdulla, Rahima
Aisa, Haji A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
By merging a high-performance liquid chromatography diode array detector (HPLC-DAD) method with high-performance thin-layer chromatography (HPTLC), an assay was developed for chemical fingerprinting and quantitative analysis of traditional medicine Majun Mupakhi ELA (MME), and constituent compounds were identified using HPLC coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS method.
In addition, the antioxidant capacity of MME was assessed based on the ability of components to scavenge radicals using in vitro method.
Using a HPLC-DAD method with HPTLC easily validated the chemical fingerprinting results and quantified three characteristic components, namely, gallic acid (1), daidzein (2), and icariin (3), in commercial MMEs.
The three compounds presented excellent regression values (R2=0.9999) in the ranges of the test and the method recovery was in the range from 100.49% to 100.68%.
The fingerprints had 27 common characteristic peaks, of which 13 were verified by rapid UHPLC-DAD-Q-Orbitrap-MS analysis.
In vitro antioxidant assays rapidly assessed and contrasted antioxidant activity or the free radical scavenging activity of the main polyphenolic classes in MMEs, and the antioxidant capacity was mostly affected by the presence of gallic acid.
Thus, this study establishes a powerful and meaningful approach for MME quality control and for assessing in vitro antioxidant activity.
American Psychological Association (APA)
Reheman, Ayinuer& Aisa, Haji A.& Ma, Qing Ling& Nijat, Dilaram& Abdulla, Rahima. 2018. Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1154283
Modern Language Association (MLA)
Reheman, Ayinuer…[et al.]. Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1154283
American Medical Association (AMA)
Reheman, Ayinuer& Aisa, Haji A.& Ma, Qing Ling& Nijat, Dilaram& Abdulla, Rahima. Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1154283
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154283