Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components
Joint Authors
Numonov, Sodik Rakhmonovich
Edirs, Salamet
Turak, Ablajan
Xin, Xuelei
Aisa, Haji A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-17
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
By using extraction yield, total polyphenolic content, antidiabetic activities (PTP-1B and α-glycosidase), and antioxidant activity (ABTS and DPPH) as indicated markers, the extraction conditions of the prescription Kursi Wufarikun Ziyabit (KWZ) were optimized by response surface methodology (RSM).
Independent variables were ethanol concentration, extraction temperature, solid-to-solvent ratio, and extraction time.
The result of RSM analysis showed that the four variables investigated have a significant effect (p<0.05) for Y1, Y2, Y3, Y4, and Y5 with R2 value of 0.9120, 0.9793, 0.9076, 0.9125, and 0.9709, respectively.
Optimal conditions for the highest extraction yield of 39.28%, PTP-1B inhibition rate of 86.21%, α-glycosidase enzymes inhibition rate of 96.56%, and ABTS inhibition rate of 77.38% were derived at ethanol concentration 50.11%, extraction temperature 72.06°C, solid-to-solvent ratio 1 : 22.73 g/mL, and extraction time 2.93 h.
On the basis of total polyphenol content of 48.44% in this optimal condition, the quantitative analysis of effective part of KWZ was characterized via UPLC method, 12 main components were identified by standard compounds, and all of them have shown good regression within the test ranges and the total content of them was 11.18%.
American Psychological Association (APA)
Edirs, Salamet& Turak, Ablajan& Numonov, Sodik Rakhmonovich& Xin, Xuelei& Aisa, Haji A.. 2017. Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1154392
Modern Language Association (MLA)
Edirs, Salamet…[et al.]. Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1154392
American Medical Association (AMA)
Edirs, Salamet& Turak, Ablajan& Numonov, Sodik Rakhmonovich& Xin, Xuelei& Aisa, Haji A.. Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1154392
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154392