Identification of the Chemical Constituents in Simiao Wan and Rat Plasma after Oral Administration by GC-MS and LC-MS
Joint Authors
Li, Yu-bo
Wang, Yu-ming
Wu, Yuanyuan
Fan, Yunshuang
Li, Yamei
Li, Lixin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-25
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Simiao Wan (SMW), an important multiherbal formula used in traditional Chinese medicine, is extensively used to treat rheumatoid arthritis.
However, the knowledge of the bioactive components of SMW remains unclear.
Thus, gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) were used to analyze the chemical constituents of volatile and nonvolatile extracts of SMW, as well as its absorbed components in rat plasma after oral SMW administration.
Identification of several compounds was enabled by comparison of retention times, MS spectra, and MS/MS spectral data with the standard substance and reference materials reported in the literature.
In the volatile extracts, GC-MS identified 26 compounds in vitro, three of which observed in blood by GC-MS.
In the nonvolatile extracts, LC-MS identified 49 compounds in SMW; 18 compounds containing 7 prototype compounds, 5 metabolites, and 6 unknown compounds were absorbed by blood.
The proposed GC-MS and LC-MS method was appropriate not only for the rapid screening and identification of multiple components of an SMW extract but also for screening its bioactive constituents in vivo.
The proposed method could be a promising tool for the quality control of other Chinese herbal medicines.
American Psychological Association (APA)
Fan, Yunshuang& Li, Yamei& Wu, Yuanyuan& Li, Lixin& Wang, Yu-ming& Li, Yu-bo. 2017. Identification of the Chemical Constituents in Simiao Wan and Rat Plasma after Oral Administration by GC-MS and LC-MS. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1154398
Modern Language Association (MLA)
Fan, Yunshuang…[et al.]. Identification of the Chemical Constituents in Simiao Wan and Rat Plasma after Oral Administration by GC-MS and LC-MS. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1154398
American Medical Association (AMA)
Fan, Yunshuang& Li, Yamei& Wu, Yuanyuan& Li, Lixin& Wang, Yu-ming& Li, Yu-bo. Identification of the Chemical Constituents in Simiao Wan and Rat Plasma after Oral Administration by GC-MS and LC-MS. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1154398
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154398