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Induction of Cell Cycle Arrest and Apoptotic Response of Head and Neck Squamous Carcinoma Cells (Detroit 562) by Caffeic Acid and Caffeic Acid Phenethyl Ester Derivative
Joint Authors
Kubina, Robert
Dziedzic, Arkadiusz
Tanasiewicz, Marta
Kabała-Dzik, Agata
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Natural polyphenols have been observed to possess antiproliferative properties.
The effects, including apoptotic potential of bioactive phenolic compounds, caffeic acid (CA) and its derivative caffeic acid phenethyl ester (CAPE), on cell proliferation and apoptosis in human head and neck squamous carcinoma cells (HNSCC) line (Detroit 562) were investigated and compared.
Cancer cells apoptosis rates and cell cycle arrests were analysed by flow cytometry.
Exposure to CA and CAPE was found to result in a dose-dependent decrease in the viability of Detroit 562 cells at different levels.
CA/CAPE treatment did significantly affect the viability of Detroit 562 cells (MTT results).
CAPE-mediated loss of viability occurred at lower doses and was more pronounced, with the concentrations which inhibit the growth of cells by 50% estimated at 201.43 μM (CA) and 83.25 μM (CAPE).
Dead Cell Assay with Annexin V labelling demonstrated that CA and CAPE treatment of Detroit 562 cells resulted in an induction of apoptosis at 50 μM and 100 μM doses.
The rise of mainly late apoptosis was observed for 100 μM dose and CA/CAPE treatment did affect the distribution of cells in G0/G1 phase.
A combination of different phenolic compounds, potentially with chemotherapeutics, could be considered as an anticancer drug.
American Psychological Association (APA)
Dziedzic, Arkadiusz& Kubina, Robert& Kabała-Dzik, Agata& Tanasiewicz, Marta. 2017. Induction of Cell Cycle Arrest and Apoptotic Response of Head and Neck Squamous Carcinoma Cells (Detroit 562) by Caffeic Acid and Caffeic Acid Phenethyl Ester Derivative. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154402
Modern Language Association (MLA)
Dziedzic, Arkadiusz…[et al.]. Induction of Cell Cycle Arrest and Apoptotic Response of Head and Neck Squamous Carcinoma Cells (Detroit 562) by Caffeic Acid and Caffeic Acid Phenethyl Ester Derivative. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154402
American Medical Association (AMA)
Dziedzic, Arkadiusz& Kubina, Robert& Kabała-Dzik, Agata& Tanasiewicz, Marta. Induction of Cell Cycle Arrest and Apoptotic Response of Head and Neck Squamous Carcinoma Cells (Detroit 562) by Caffeic Acid and Caffeic Acid Phenethyl Ester Derivative. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154402
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154402