Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis
Joint Authors
Xie, Shu-wu
Zhong, Ruihua
Ma, Aying
Li, Zhao
Li, Guoting
Qu, Lijuan
Chen, Ping
Lu, Zhigang
Zhou, Jieyun
Guo, Xiangjie
Qian, Yueying
Zhu, Yan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-04
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
This study aimed to investigate the antifertility effect of Juniperus sabina fruit on male rats and its possible mechanism, and hence it might be developed as a potential nonhormonal male contraceptive.
Male rats were intragastrically fed for consecutive 8-week and 4-week recovery with the fruit of J.
Sabina, and sperm maturation, serum testosterone level, and histopathology were analyzed.
Epididymal epithelial cell culture was prepared for detection of podophyllotoxin activities.
Furthermore, cell proliferation, transmission electron microscopy, Annexin V/Propidium iodide, TUNEL, RT-PCR, ELISA, and western blotting were examined.
The results showed that rat sperm motility and fertility were remarkably declined after feeding the fruit.
Moreover, the fruit targeted the epididymis rather than the testis.
After 4-week recovery, more than half of the male rats resumed normal fertility.
It was found that podophyllotoxin significantly inhibited epididymal epithelial cell proliferation, promoted cell apoptosis, and increased the mRNA and protein levels of TNF-α and the expression levels of cytochrome c, caspase-8, caspase-9, and caspase-3.
Our findings suggest that the fruit of J.
sabina could inhibit male rat sperm maturation and fertility.
The potential mechanism might be related to podophyllotoxin, inducing epididymal epithelial cell apoptosis through TNF-α and caspase signaling pathway.
American Psychological Association (APA)
Xie, Shu-wu& Li, Guoting& Qu, Lijuan& Zhong, Ruihua& Chen, Ping& Lu, Zhigang…[et al.]. 2017. Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1154421
Modern Language Association (MLA)
Xie, Shu-wu…[et al.]. Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1154421
American Medical Association (AMA)
Xie, Shu-wu& Li, Guoting& Qu, Lijuan& Zhong, Ruihua& Chen, Ping& Lu, Zhigang…[et al.]. Podophyllotoxin Extracted from Juniperus sabina Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1154421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154421