Green Tea Polyphenols and Padma Hepaten Inhibit Candida albicans Biofilm Formation
Joint Authors
Feldman, Mark
Steinberg, Doron
Farkash, Yosi
Ginsburg, Isaac
Shalish, Miriam
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Candida albicans (C.
albicans) is the most prevalent opportunistic human pathogenic fungus and can cause mucosal membrane infections and invade the blood.
In the oral cavity, it can ferment dietary sugars, produce organic acids and therefore has a role in caries development.
In this study, we examined whether the polyphenol rich extractions Polyphenon from green tea (PPFGT) and Padma Hepaten (PH) can inhibit the caries-inducing properties of C.
albicans.
Biofilms of C.
albicans were grown in the presence of PPFGT and PH.
Formation of biofilms was tested spectrophotometrically after crystal violet staining.
Exopolysaccharides (EPS) secretion was quantified using confocal scanning laser microscopy (CSLM).
Treated C.
albicans morphology was demonstrated using scanning electron microscopy (SEM).
Expression of virulence-related genes was tested using qRT-PCR.
Development of biofilm was also tested on an orthodontic surface (Essix) to assess biofilm inhibition ability on such appliances.
Both PPFGT and PH dose-dependently inhibited biofilm formation, with no inhibition on planktonic growth.
The strongest inhibition was obtained using the combination of the substances.
Crystal violet staining showed a significant reduction of 45% in biofilm formation using a concentration of 2.5mg/ml PPFGT and 0.16mg/ml PH.
A concentration of 1.25 mg/ml PPFGT and 0.16 mg/ml PH inhibited candidal growth by 88% and EPS secretion by 74% according to CSLM.
A reduction in biofilm formation and in the transition from yeast to hyphal morphotype was observed using SEM.
A strong reduction was found in the expression of hwp1, eap1, and als3 virulence associated genes.
These results demonstrate the inhibitory effect of natural PPFGT polyphenolic extraction on C.
albicans biofilm formation and EPS secretion, alone and together with PH.
In an era of increased drug resistance, the use of phytomedicine to constrain biofilm development, without killing host cells, may pave the way to a novel therapeutic concept, especially in children as orthodontic patients.
American Psychological Association (APA)
Farkash, Yosi& Feldman, Mark& Ginsburg, Isaac& Steinberg, Doron& Shalish, Miriam. 2018. Green Tea Polyphenols and Padma Hepaten Inhibit Candida albicans Biofilm Formation. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1154441
Modern Language Association (MLA)
Farkash, Yosi…[et al.]. Green Tea Polyphenols and Padma Hepaten Inhibit Candida albicans Biofilm Formation. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1154441
American Medical Association (AMA)
Farkash, Yosi& Feldman, Mark& Ginsburg, Isaac& Steinberg, Doron& Shalish, Miriam. Green Tea Polyphenols and Padma Hepaten Inhibit Candida albicans Biofilm Formation. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1154441
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154441