Dandelion Chloroform Extract Promotes Glucose Uptake via the AMPKGLUT4 Pathway in L6 Cells
Joint Authors
Yang, Xinzhou
Shen, Jinhua
Liu, Qing-Hua
Zhao, Ping
Ming, Qian
Xiong, Mingrui
Song, Guanjun
Tan, Li
Tian, Di
Liu, Jia
Huang, Zhao
Ma, Jingyi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The number of patients with type 2 diabetes mellitus (T2DM) is increasing rapidly worldwide.
Glucose transporter 4 (GLUT4) is one of the main proteins that transport blood glucose into the cells and is a target in the treatment of T2DM.
In this study, we investigated the mechanism of action of dandelion chloroform extract (DCE) on glucose uptake in L6 cells.
The glucose consumption of L6 cell culture supernatant was measured by a glucose uptake assay kit, and the dynamic changes of intracellular GLUT4 and calcium (Ca2+) levels were monitored by laser scanning confocal microscopy in L6 cell lines stably expressing IRAP-mOrange.
The GLUT4 fusion with the plasma membrane (PM) was traced via myc-GLUT4-mOrange.
GLUT4 expression and AMP-activated protein kinase (AMPK), protein kinase B (PKB/Akt), protein kinase C (PKC), and phosphorylation levels were determined by performing western blotting.
GLUT4 mRNA expression was detected by real-time PCR.
DCE up-regulated GLUT4 expression, promoted GLUT4 translocation and fusion to the membrane eventually leading to glucose uptake, and induced AMPK phosphorylation in L6 cells.
The AMPK inhibitory compound C significantly inhibited DCE-induced GLUT4 expression and translocation while no inhibitory effect was observed by the phosphatidylinositol 3-kinase (PI3K) inhibitor Wortmannin and PKC inhibitor Gö6983.
These data suggested that DCE promoted GLUT4 expression and transport to the membrane through the AMPK signaling pathway, thereby stimulating GLUT4 fusion with PM to enhance glucose uptake in L6 cells.
DCE-induced GLUT4 translocation was also found to be Ca2+-independent.
Together, these findings indicate that DCE could be a new hypoglycemic agent for the treatment of T2DM.
American Psychological Association (APA)
Zhao, Ping& Ming, Qian& Xiong, Mingrui& Song, Guanjun& Tan, Li& Tian, Di…[et al.]. 2018. Dandelion Chloroform Extract Promotes Glucose Uptake via the AMPKGLUT4 Pathway in L6 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1154454
Modern Language Association (MLA)
Zhao, Ping…[et al.]. Dandelion Chloroform Extract Promotes Glucose Uptake via the AMPKGLUT4 Pathway in L6 Cells. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1154454
American Medical Association (AMA)
Zhao, Ping& Ming, Qian& Xiong, Mingrui& Song, Guanjun& Tan, Li& Tian, Di…[et al.]. Dandelion Chloroform Extract Promotes Glucose Uptake via the AMPKGLUT4 Pathway in L6 Cells. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1154454
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154454