Erzhi Formula Extracts Reverse Renal Injury in Diabetic Nephropathy Rats by Protecting the Renal Podocytes
Joint Authors
Jiang, Jun
Yin, Jiangning
Liu, Xiang
Wang, Huajun
Lu, Guoyuan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-23
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Podocytes injury was a crucial factor resulting in diabetic nephropathy (DN).
Erzhi formula extract (EZF) was a clinical effective Chinese medicine on DN, but its mechanism was unclear.
In this study, the main compounds of EZF and their pharmacokinetics in rat were detected by HPLC-MS/MS.
And then, blood glucose, urine protein, renal index, renal microstructural (HE/PAS staining), inflammatory factors (IL-β, TNF-α, IL-6), and protein/mRNA expression related to the function of podocyte (CD2AP and Podocin) in DN rats were investigated after the oral administration of EZF.
The concentrations of specnuezhenide and wedelolactone in rat kidney were 7.19 and 0.057 mg/kg, respectively.
The Tmax of specnuezhenide and wedelolactone were 2.0 and 1.50 h, respectively.
Their Cmax were, respectively, 30.24 ± 2.68 and 6.39 ± 0.05 μg/L.
Their AUC(0-∞) were 123.30 ± 2.68 and 16.56 ± 0.98 μg/L⁎h, respectively.
Compared with the model group, the blood glucose and the 24-hour urinary protein were significantly decreased (P < 0.05) after 16 weeks’ treatment of EZF.
The expressions of Podocin and CD2AP protein/mRNA were increased (P < 0.
05).
The deteriorate of glomerular morphology was alleviated under the treatment of EZF.
EZF prominently decreased the levels of inflammatory factors (P < 0.05).
MDA was significantly decreased (P < 0.05) with the significant increase of SOD activity (P < 0.05) in EZF groups.
All the results proved that EZF repaired glomerular mesangial matrix, protected renal tubule, and improved renal function in DN rats by upregulating the expression of Podocin and CD2AP protein/mRNA in podocytes.
American Psychological Association (APA)
Jiang, Jun& Yin, Jiangning& Liu, Xiang& Wang, Huajun& Lu, Guoyuan. 2018. Erzhi Formula Extracts Reverse Renal Injury in Diabetic Nephropathy Rats by Protecting the Renal Podocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1154461
Modern Language Association (MLA)
Jiang, Jun…[et al.]. Erzhi Formula Extracts Reverse Renal Injury in Diabetic Nephropathy Rats by Protecting the Renal Podocytes. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1154461
American Medical Association (AMA)
Jiang, Jun& Yin, Jiangning& Liu, Xiang& Wang, Huajun& Lu, Guoyuan. Erzhi Formula Extracts Reverse Renal Injury in Diabetic Nephropathy Rats by Protecting the Renal Podocytes. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1154461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154461