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Chemical Composition and Antifungal In Vitro and In Silico, Antioxidant, and Anticholinesterase Activities of Extracts and Constituents of Ouratea fieldingiana (DC.) Baill
Joint Authors
de Morais, Selene Maia
Silva, Cecília R.
Júnior, Hélio V. N.
Alves, Daniela Ribeiro
Rodrigues, Ana Livya Moreira
Nascimento, José Eranildo Teles do
Lisboa, Daniele Silva de
Liberato, Hortência Ribeiro
Falcão, Maria José Cajazeiras
Braz Filho, Raimundo
Paula Junior, Valdir Ferreira de
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Ouratea fieldingiana (Gardner) Engl is popularly used for wound healing.
This study describes the main chemical compounds present in extracts of O.
fieldingiana and evaluates their biological potential by investigating antifungal, antioxidant, and anticholinesterase activities.
The action mechanism of main antifungal compound was investigated by molecular docking using the enzyme sterol 14-α demethylase, CYP51, required for ergosterol biosynthesis.
The seeds and leaves were extracted with ethanol in a Soxhlet apparatus and by maceration, respectively.
Both extracts were subjected to silica gel column chromatography for isolation of main constituents, followed by purification in sephadex.
The structures of compounds were established by 1H and 13C-NMR spectroscopy and identified by comparison with literature data as amentoflavone and kaempferol 3-O-rutinoside, respectively.
The antioxidant activities of the extracts were determined by the DPPH and ABTS free radical inhibition methods.
In general, the extracts with the highest antioxidant activity corresponded to those with higher content of phenolic compounds and flavonoids.
The ethanol extracts and two isolated compounds presented relevant antifungal activity against several Candida strains.
The in silico findings revealed that the compound amentoflavone coupled with the CYP450 protein due to the low energy stabilization (-9.39 kcal/mol), indicating a possible mechanism of action by inhibition of the ergosterol biosynthesis of Candida fungi.
American Psychological Association (APA)
Nascimento, José Eranildo Teles do& Rodrigues, Ana Livya Moreira& Lisboa, Daniele Silva de& Liberato, Hortência Ribeiro& Falcão, Maria José Cajazeiras& Silva, Cecília R.…[et al.]. 2018. Chemical Composition and Antifungal In Vitro and In Silico, Antioxidant, and Anticholinesterase Activities of Extracts and Constituents of Ouratea fieldingiana (DC.) Baill. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1154463
Modern Language Association (MLA)
Nascimento, José Eranildo Teles do…[et al.]. Chemical Composition and Antifungal In Vitro and In Silico, Antioxidant, and Anticholinesterase Activities of Extracts and Constituents of Ouratea fieldingiana (DC.) Baill. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1154463
American Medical Association (AMA)
Nascimento, José Eranildo Teles do& Rodrigues, Ana Livya Moreira& Lisboa, Daniele Silva de& Liberato, Hortência Ribeiro& Falcão, Maria José Cajazeiras& Silva, Cecília R.…[et al.]. Chemical Composition and Antifungal In Vitro and In Silico, Antioxidant, and Anticholinesterase Activities of Extracts and Constituents of Ouratea fieldingiana (DC.) Baill. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1154463
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154463