Methanol Extract of Holarrhena antidysenterica Inhibits the Growth of Human Oral Squamous Cell Carcinoma Cells and Osteoclastogenesis of Bone Marrow Macrophages
Joint Authors
Park, Kwang-Kyun
Park, Junhee
Tilakaratne, W. M.
Yoon, Heein
Kim, Jin
Bandara, N. Champika
Bandara, B. M. R.
Chung, Won-Yoon
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Oral squamous cell carcinoma (OSCC) frequently invades mandibular bone, and outcomes for treatment with surgical resection are typically poor, ultimately resulting in death.
Holarrhena antidysenterica L.
(Apocynaceae), distributed throughout Sri Lanka and India, has been used as a folk remedy to treat various diseases.
Treatment with methanol extract of H.
antidysenterica bark (HABE) inhibited cell viability and BrdU incorporation and induced apoptotic cell death in Ca9-22 gingival and HSC-3 tongue SCC cells.
Flow cytometric analysis indicated that HABE treatment preferentially induces apoptotic cell death via increasing the sub-G1 peak in Ca9-22 cells and cell cycle arrest at the G1 phase in HSC-3 cells.
HABE treatment in the presence of zVAD-fmk, a pan-caspase inhibitor, rescued cell viabilities in both OSCC cell lines.
The ratio of Bax to Bcl-2 increased with reductions in the Bcl-2 protein expression, and the activation of caspase 3 and subsequent cleavage of PARP was detected in HABE-treated Ca9-22 and HSC-3 cells.
Furthermore, HABE treatment at noncytotoxic concentrations inhibited osteoclast formation in RANKL-stimulated bone marrow macrophages.
Taken together, HABE possesses the inhibitory activity on the growth of OSCC cells and antiosteoclastogenic activity.
Therefore, HABE may be a promising alternative and complementary agent for preventing and treating OSCC.
American Psychological Association (APA)
Yoon, Heein& Park, Junhee& Park, Kwang-Kyun& Kim, Jin& Bandara, N. Champika& Bandara, B. M. R.…[et al.]. 2017. Methanol Extract of Holarrhena antidysenterica Inhibits the Growth of Human Oral Squamous Cell Carcinoma Cells and Osteoclastogenesis of Bone Marrow Macrophages. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1154492
Modern Language Association (MLA)
Yoon, Heein…[et al.]. Methanol Extract of Holarrhena antidysenterica Inhibits the Growth of Human Oral Squamous Cell Carcinoma Cells and Osteoclastogenesis of Bone Marrow Macrophages. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1154492
American Medical Association (AMA)
Yoon, Heein& Park, Junhee& Park, Kwang-Kyun& Kim, Jin& Bandara, N. Champika& Bandara, B. M. R.…[et al.]. Methanol Extract of Holarrhena antidysenterica Inhibits the Growth of Human Oral Squamous Cell Carcinoma Cells and Osteoclastogenesis of Bone Marrow Macrophages. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1154492
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154492