Wu-Mei-Wan Reduces Insulin Resistance via Inhibition of NLRP3 Inflammasome Activation in HepG2 Cells
Joint Authors
Lu, Fuer
Fang, Ke
Yang, Xueping
Li, Lingli
Dong, Ruolan
Li, Jingbin
Dong, Hui
Yi, Ping
Chen, Guang
Huang, Zhaoyi
Zhao, Yan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Wu-Mei-Wan (WMW) is a Chinese herbal formula used to treat type 2 diabetes.
In this study, we aimed to explore the effects and mechanisms of WMW on insulin resistance in HepG2 cells.
HepG2 cells were pretreated with palmitate (0.25 mM) to impair the insulin signaling pathway.
Then, they were treated with different doses of WMW-containing medicated serum and stimulated with 100 nM insulin.
Results showed that palmitate could reduce the glucose consumption rate in HepG2 cells and impair insulin signaling related to phosphorylation of insulin receptor (IR) and insulin receptor substrate-1 (IRS-1), thereby regulating the downstream signaling pathways.
However, medicated serum of WMW restored impaired insulin signaling, upregulated the expression of phospho-IR (pIR), phosphatidylinositol 3-kinase p85 subunit, phosphoprotein kinase B, and glucose transporter 4, and decreased IRS serine phosphorylation.
In addition, it decreased the expression of interleukin-1β and tumor necrosis factor-α, which are the key proinflammatory cytokines involved in insulin resistance; besides, it reduced the expression of NLRP3 inflammasome.
These results suggested that WMW could alleviate palmitate-induced insulin resistance in HepG2 cells via inhibition of NLRP3 inflammasome and reduction of proinflammatory cytokine production.
American Psychological Association (APA)
Yang, Xueping& Li, Lingli& Fang, Ke& Dong, Ruolan& Li, Jingbin& Zhao, Yan…[et al.]. 2017. Wu-Mei-Wan Reduces Insulin Resistance via Inhibition of NLRP3 Inflammasome Activation in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154497
Modern Language Association (MLA)
Yang, Xueping…[et al.]. Wu-Mei-Wan Reduces Insulin Resistance via Inhibition of NLRP3 Inflammasome Activation in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154497
American Medical Association (AMA)
Yang, Xueping& Li, Lingli& Fang, Ke& Dong, Ruolan& Li, Jingbin& Zhao, Yan…[et al.]. Wu-Mei-Wan Reduces Insulin Resistance via Inhibition of NLRP3 Inflammasome Activation in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154497
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154497