Phosphatidylinositide 3-Kinase Contributes to the Anti-Inflammatory Effect of Abutilon crispum L. Medik Methanol Extract
Joint Authors
Kim, Jong-Hoon
Shin, Kon Kuk
Park, Sang Hee
Hunto, Stephanie Triseptya
Kim, Han Gyung
Oh, Junsang
Hossain, Mohammad Amjad
Rho, Ho Sik
Cho, Jae Youl
Sung, Gi-Ho
Lee, Jongsung
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Abutilon crispum L.
Medik, better known as bladdermallow, is used as a traditional remedy in India, for its anti-inflammatory effect due to its high content of flavonoids.
However, research about its anti-inflammatory effect at the molecular level has not been performed.
In this study, we aimed to investigate the mechanism of Abutilon crispum methanol extract (Ac-ME) in inhibiting the inflammatory response by conducting several experiments including cellular and molecular assays.
Ac-ME inhibited the production of nitric oxide (NO) in RAW264.7 cells during treatment of LPS and Pam3CSK4 without exhibiting cytotoxicity.
Ac-ME also suppressed the mRNA expression of inducible nitric oxide (iNOS) and proinflammatory cytokines such as interleukin (IL)-1β and IL-6.
Moreover, Ac-ME was shown to inhibit the NF-κB pathway, according to the luciferase reporter gene assay performed with a NF-κB-Luc construct containing NF-κB-binding promoter regions under MyD88 and TRIF overexpression conditions, and immunoblotting analysis by determining the phospho-form levels of IκBα, IKKα/β, and p85, a regulatory domain of phosphatidylinositide 3-kinase (PI3K).
Finally, we observed that the level of phospho-p85 induced by the overexpression of spleen tyrosine kinase (Syk) and Src was decreased by Ac-ME at 200 μg/ml.
Therefore, these results suggest that Ac-ME has an anti-inflammatory effect by targeting PI3K in the NF-κB signaling pathway.
American Psychological Association (APA)
Hunto, Stephanie Triseptya& Shin, Kon Kuk& Kim, Han Gyung& Park, Sang Hee& Oh, Junsang& Sung, Gi-Ho…[et al.]. 2018. Phosphatidylinositide 3-Kinase Contributes to the Anti-Inflammatory Effect of Abutilon crispum L. Medik Methanol Extract. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1154501
Modern Language Association (MLA)
Hunto, Stephanie Triseptya…[et al.]. Phosphatidylinositide 3-Kinase Contributes to the Anti-Inflammatory Effect of Abutilon crispum L. Medik Methanol Extract. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1154501
American Medical Association (AMA)
Hunto, Stephanie Triseptya& Shin, Kon Kuk& Kim, Han Gyung& Park, Sang Hee& Oh, Junsang& Sung, Gi-Ho…[et al.]. Phosphatidylinositide 3-Kinase Contributes to the Anti-Inflammatory Effect of Abutilon crispum L. Medik Methanol Extract. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1154501
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154501