Astragalus Granule Prevents Ca2+ Current Remodeling in Heart Failure by the Downregulation of CaMKII
Joint Authors
Li, Li-na
Liu, Weihong
Zhou, Mingxue
Li, Yan
Li, Sinai
Nong, Yibing
Gao, Qun
Liu, Jing
Cui, Xiaoyun
Wan, Jie
Lu, Jinjin
Sun, Mingjie
Wu, Qian
Shi, Xiaolu
Cui, Haifeng
Lin, Qian
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Astragalus was broadly used for treating heart failure (HF) and arrhythmias in East Asia for thousands of years.
Astragalus granule (AG), extracted from Astragalus, shows beneficial effect on the treatment of HF in clinical research.
We hypothesized that administration of AG prevents the remodeling of L-type Ca2+ current (ICa-L) in HF mice by the downregulation of Ca2+/calmodulin-dependent protein kinase II (CaMKII).
Methods.
HF mice were induced by thoracic aortic constriction (TAC).
After 4 weeks of AG treatment, cardiac function and QT interval were evaluated.
Single cardiac ventricular myocyte was then isolated and whole-cell patch clamp was used to record action potential (AP) and ICa-L.
The expressions of L-type calcium channel alpha 1C subunit (Cav1.2), CaMKII, and phosphorylated protein kinase A (p-PKA) were examined by western blot.
Results.
The failing heart manifested distinct electrical remodeling including prolonged repolarization time and altered ICa-L kinetics.
AG treatment attenuated this electrical remodeling, supported by AG-related shortened repolarization time, decreased peak ICa-L, accelerated ICa-L inactivation, and positive frequency-dependent ICa-L facilitation.
In addition, AG treatment suppressed the overexpression of CaMKII, but not p-PKA, in the failing heart.
Conclusion.
AG treatment protected the failing heart against electrical remodeling and ICa-L remodeling by downregulating CaMKII.
American Psychological Association (APA)
Li, Sinai& Nong, Yibing& Gao, Qun& Liu, Jing& Li, Yan& Cui, Xiaoyun…[et al.]. 2017. Astragalus Granule Prevents Ca2+ Current Remodeling in Heart Failure by the Downregulation of CaMKII. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154564
Modern Language Association (MLA)
Li, Sinai…[et al.]. Astragalus Granule Prevents Ca2+ Current Remodeling in Heart Failure by the Downregulation of CaMKII. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154564
American Medical Association (AMA)
Li, Sinai& Nong, Yibing& Gao, Qun& Liu, Jing& Li, Yan& Cui, Xiaoyun…[et al.]. Astragalus Granule Prevents Ca2+ Current Remodeling in Heart Failure by the Downregulation of CaMKII. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154564
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154564