Effects of Jiawei Shaoyao-Gancao Decoction and Its Drug-Containing Serum on Proliferation, Apoptosis, and Ultrastructure of Human Adenomyosis Foci Cells

Joint Authors

Zeng, Yuyan
Li, Kun-Yin

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-09-12

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

Objective.

The present study aimed to investigate the effects of Jiawei Shaoyao-Gancao Decoction (JSGD) and its drug-containing serum (CDS) on the proliferation, apoptosis, and ultrastructure of human adenomyosis foci cells.

Methods.

Primary cultures of human adenomyosis foci cells were prepared from hard uterine lesions of adenomyosis patients.

The cells were treated with JSGD (10 and 20 mg/ml), CDS, and mifepristone (MIF) for 24 or 48 h.

Cell proliferation was detected using CCK-8 assay, cell apoptosis was measured by flow cytometry, and the cell ultrastructure was observed by transmission electron microscopy (TEM).

Results.

JSGD and CDS significantly induced cell apoptosis and inhibited cell proliferation for 24 h or 48 h, in which the effects of JSGD were in a dose-dependent manner.

The effect of CDS for 24 h was higher than that of CDS for 48 h.

Moreover, JSGD and CDS treatments induced marked apoptosis in adenomyosis foci cells, characterized by nucleus chromatin, condensation, fragmentation, mitochondria and endoplasmic swelling, and autophagy-lysosome.

Conclusions.

JSGD and CDS can suppress proliferation and induce apoptosis in adenomyosis foci cells, through altering their ultrastructure.

The results provided support for JSGD and CDS in the treatment of adenomyosis and gained further insight into the effect of this prescription.

American Psychological Association (APA)

Zeng, Yuyan& Li, Kun-Yin. 2017. Effects of Jiawei Shaoyao-Gancao Decoction and Its Drug-Containing Serum on Proliferation, Apoptosis, and Ultrastructure of Human Adenomyosis Foci Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1154602

Modern Language Association (MLA)

Zeng, Yuyan& Li, Kun-Yin. Effects of Jiawei Shaoyao-Gancao Decoction and Its Drug-Containing Serum on Proliferation, Apoptosis, and Ultrastructure of Human Adenomyosis Foci Cells. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1154602

American Medical Association (AMA)

Zeng, Yuyan& Li, Kun-Yin. Effects of Jiawei Shaoyao-Gancao Decoction and Its Drug-Containing Serum on Proliferation, Apoptosis, and Ultrastructure of Human Adenomyosis Foci Cells. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1154602

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1154602