Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway
Joint Authors
Choi, Yung Hyun
Kim, Seung-Hyung
Irfan, Muhammad
Park, Sun Haeng
Kim, Dong-Seon
Kim, Ho Kyoung
Sung, Yoon-Young
Rhee, Man Hee
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Morus alba L.
(MAL) extract has been used in traditional medicine for its cardioprotective and antiplatelet effects, while another herbal remedy, Schisandra chinensis (SCC), has been reported to have anti-inflammatory and antioxidant properties.
We evaluated underlying cellular changes exerted by extracts of these plants on platelet function and effects of SCC + MAL on in vivo thrombus formation using AV shunt and tail thrombosis-length models in rats.
In vitro platelet aggregation, granule secretion, and Ca2+i release assays were carried out.
The activation of integrin αIIbβ3 and phosphorylation of downstream signaling molecules, including MAPK and Akt, were investigated using cytometry and immunoblotting, respectively.
Scanning electron microscopy (SEM) was used to evaluate changes in platelet shape and HPLC analysis was carried out to identify the marker compounds in SCC + MAL mixture.
In vivo thrombus weight and average length of tail thrombosis were significantly decreased by SCC + MAL.
In vitro platelet aggregation, granule secretion, Ca2+i release, and integrin αIIbβ3 activation were notably inhibited.
SCC + MAL markedly reduced the phosphorylation of MAPK pathway factors along with Akt.
HPLC analysis identified four marker compounds: isoquercitrin, astragalin, schizandrol A, and gomisin A.
The extracts exerted remarkable synergistic effects as natural antithrombotic and antiplatelet agent and a potent drug candidate for treating cardiovascular diseases.
American Psychological Association (APA)
Kim, Dong-Seon& Irfan, Muhammad& Sung, Yoon-Young& Kim, Seung-Hyung& Park, Sun Haeng& Choi, Yung Hyun…[et al.]. 2017. Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154612
Modern Language Association (MLA)
Kim, Dong-Seon…[et al.]. Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154612
American Medical Association (AMA)
Kim, Dong-Seon& Irfan, Muhammad& Sung, Yoon-Young& Kim, Seung-Hyung& Park, Sun Haeng& Choi, Yung Hyun…[et al.]. Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154612
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154612