Active Ingredients of Epimedii Folium and Ligustri Lucidi Fructus Balanced GRHSP90 to Improve the Sensitivity of Asthmatic Rats to Budesonide
Joint Authors
Liu, RenHui
Xu, LiPing
Nian, HongLei
Yang, XinWei
Wang, XiuJuan
Chen, Yuheng
Li, Xiaoxi
Tang, Xiufeng
Yang, Yan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-11
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
This study aimed to investigate the possible molecular mechanisms of active ingredients of Epimedii Folium (EF) and Ligustri Lucidi Fructus (LLF) combined with Budesonide (Bun) in asthmatic rats.
Rats were divided into 5 groups, including normal group, asthma model group, Bun group, group of active ingredients of EL and LLF (EL), and group of coadministration of Bun with EL (Bun&EL).
The asthmatic model was prepared by ovalbumin sensitizing and challenging.
Lymphocyte apoptosis, GR protein and binding, and the protein and mRNA of GRα, GRβ, and HSP90 were tested.
The results showed that Bun&EL ① markedly increased lymphocyte apoptosis, GR and HSP90 protein, and GR binding in BALF and ② enhanced the expressions of GRα and HSP90 and the ratio of GRα to GRβ or to HSP90 both in protein and in mRNA levels in lung, ③ while decrease occurred in GRβ mRNA and the mRNA ratio of GRβ to HSP90 compared with asthma or Bun group.
Moreover, there was a significant correlation between GRα and GRβ in protein level, or between GRα and HSP90 both in protein and in mRNA levels.
EL may effectively enhance the sensitivity of asthmatic rats to Bun via balancing GR/HSP90.
And these findings will be beneficial for the treatment of asthma in the future.
American Psychological Association (APA)
Tang, Xiufeng& Li, Xiaoxi& Nian, HongLei& Yang, Yan& Chen, Yuheng& Wang, XiuJuan…[et al.]. 2017. Active Ingredients of Epimedii Folium and Ligustri Lucidi Fructus Balanced GRHSP90 to Improve the Sensitivity of Asthmatic Rats to Budesonide. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1154637
Modern Language Association (MLA)
Tang, Xiufeng…[et al.]. Active Ingredients of Epimedii Folium and Ligustri Lucidi Fructus Balanced GRHSP90 to Improve the Sensitivity of Asthmatic Rats to Budesonide. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-16.
https://search.emarefa.net/detail/BIM-1154637
American Medical Association (AMA)
Tang, Xiufeng& Li, Xiaoxi& Nian, HongLei& Yang, Yan& Chen, Yuheng& Wang, XiuJuan…[et al.]. Active Ingredients of Epimedii Folium and Ligustri Lucidi Fructus Balanced GRHSP90 to Improve the Sensitivity of Asthmatic Rats to Budesonide. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1154637
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154637