Cardioprotective Effect of Danshensu against IschemicReperfusion Injury via c-Subunit of ATP Synthase Inhibition
Joint Authors
Gao, Qing
Jiang, Xijuan
Zhao, JingYi
Fan, Zixuan
Bao, Jiadi
Sun, Dawei
Li, Huhu
Sun, Chun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Mitochondrial permeability transition pore (MPTP) opening is the main culprit of ischemic/reperfusion (IR) injury.
It is reported that c-subunit of ATP synthase is the core component of MPTP.
Danshensu (DSS), a monomer isolated from the traditional Chinese herb Danshen, has showed cardioprotective effect against IR injury through unknown mechanism.
In this study, rat hearts were suspended in Langendorff instrument and perfused with Krebs-Henseleit (KH) buffer containing DSS for 60 minutes, followed by 30 minutes of global ischemia.
Parameters including heart rate, left ventricular developed pressure, and the rate of left ventricle diastolic pressure change were recorded to assess their cardiac function.
All these indexes were improved in DSS group.
The rate of cardiomyocytes apoptosis and MPTP opening were both inhibited in DSS group.
In addition, DSS administration leads to downregulation of c-subunit of ATP synthase in both mRNA and protein levels.
Consistently, when c-subunit of ATP synthase was overexpressed in H9C2 cells through pcDNA3/5G1 plasmid transfection, MPTP opening was enhanced when the cardioprotective effect of DSS also tapers.
In conclusion, DSS could alleviate cardiac IR injury via inhibiting c-subunit of ATP synthase expression.
American Psychological Association (APA)
Gao, Qing& Zhao, JingYi& Fan, Zixuan& Bao, Jiadi& Sun, Dawei& Li, Huhu…[et al.]. 2017. Cardioprotective Effect of Danshensu against IschemicReperfusion Injury via c-Subunit of ATP Synthase Inhibition. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154641
Modern Language Association (MLA)
Gao, Qing…[et al.]. Cardioprotective Effect of Danshensu against IschemicReperfusion Injury via c-Subunit of ATP Synthase Inhibition. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1154641
American Medical Association (AMA)
Gao, Qing& Zhao, JingYi& Fan, Zixuan& Bao, Jiadi& Sun, Dawei& Li, Huhu…[et al.]. Cardioprotective Effect of Danshensu against IschemicReperfusion Injury via c-Subunit of ATP Synthase Inhibition. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154641
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154641