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Antileishmanial Activity of Handroanthus serratifolius (Vahl) S. Grose (Bignoniaceae)
Joint Authors
Costa, Erica Vanessa Souza
Brígido, Heliton Patrick Cordovil
Silva, João Victor da Silva e
Coelho-Ferreira, Marlia Regina
Brandão, Geraldo Célio
Dolabela, Maria Fâni
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
This study aimed to evaluate the leishmanicidal activity of ethanol extract, fractions, and isolated substance from Handroanthus serratifolius against Leishmania amazonensis.
Furthermore, this activity was related to cytotoxicity, and the selectivity index was determined.
The ethanol extract was obtained by maceration of the stem powder, and the extract was subjected to fractionation on chromatographic column.
The lapachol was obtained by acid base extraction followed by purification in chromatographic column.
The antipromastigote activity and cytotoxicity tests were carried out by the cell viability method (MTT).
Modified THP-1 cells were infected with L.
amazonensis promastigotes and treated for 24 h with different concentrations of the extract, fractions, and lapachol.
The ethanol extract, dichloromethane, and ethyl acetate fractions were not active against promastigotes (IC50 > 200 μg/mL) or cytotoxic (CC50 > 500 μg/mL), and the selectivity index (SI) was greater than 2.5.
The ethyl acetate fraction was active only in promastigotes; it is not cytotoxic (CC50 > 500 μg/mL, SI > 5).
The lapachol was selectively active only against amastigote (IS > 2.5, CC50 > 500 μg/mL).
In summary, lapachol and ethyl acetate fraction are promising against amastigote and promastigote forms, respectively.
American Psychological Association (APA)
Costa, Erica Vanessa Souza& Brígido, Heliton Patrick Cordovil& Silva, João Victor da Silva e& Coelho-Ferreira, Marlia Regina& Brandão, Geraldo Célio& Dolabela, Maria Fâni. 2017. Antileishmanial Activity of Handroanthus serratifolius (Vahl) S. Grose (Bignoniaceae). Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1154652
Modern Language Association (MLA)
Costa, Erica Vanessa Souza…[et al.]. Antileishmanial Activity of Handroanthus serratifolius (Vahl) S. Grose (Bignoniaceae). Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1154652
American Medical Association (AMA)
Costa, Erica Vanessa Souza& Brígido, Heliton Patrick Cordovil& Silva, João Victor da Silva e& Coelho-Ferreira, Marlia Regina& Brandão, Geraldo Célio& Dolabela, Maria Fâni. Antileishmanial Activity of Handroanthus serratifolius (Vahl) S. Grose (Bignoniaceae). Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1154652
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154652