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In Vivo and In Vitro Hepatoprotective Effects of Geranium koreanum Methanolic Extract via Downregulation of MAPKCaspase-3 Pathway
Joint Authors
Nam, Hyeon Hwa
Choo, Byung Kil
Akanda, Md Rashedunnabi
Kim, In-Shik
Ahn, Dongchoon
Tae, Hyun-Jin
Tian, Weishun
Park, Byung-Yong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Geranium koreanum (GK) is an indigenous Chinese herbal medicine widely used for the treatment of various inflammation and liver disorders.
However, the exact mechanism of action of GK remains unknown.
This study aimed to investigate the protective effect and related molecular mechanism of GK on NaAsO2-induced cytotoxicity in HepG2 cells and liver damage in mice.
The cytoprotective role of GK was assessed on HepG2 cells using MTT assay.
Oxidative stress and lactate dehydrogenase levels were measured with ROS and LDH assay.
Histopathology and serum enzymes levels were estimated.
The molecular mechanism was evaluated by qPCR and immunoblotting to ensure the hepatoprotective role of GK against NaAsO2 intoxication in mice.
We found cotreatment with GK significantly attenuated NaAsO2-induced cell viability loss, intracellular ROS, and LDH release.
Hepatic histopathology and serum biochemical parameters, ALT, and AST were notably improved by cotreatment with GK.
Beside, GK markedly altered both mRNA and protein expression level of MAPK.
The proapoptotic and antiapoptotic protein Bax/Bcl-2 ratio was significantly regulated by GK.
Moreover, GK remarkably suppressed the postapoptotic transcription protein cleaved caspase-3 expression.
The present study reveals that GK possesses hepatoprotective activity which is probably involved in the modulation of the MAPK/caspase-3 pathway.
American Psychological Association (APA)
Akanda, Md Rashedunnabi& Kim, In-Shik& Ahn, Dongchoon& Tae, Hyun-Jin& Tian, Weishun& Nam, Hyeon Hwa…[et al.]. 2017. In Vivo and In Vitro Hepatoprotective Effects of Geranium koreanum Methanolic Extract via Downregulation of MAPKCaspase-3 Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1154666
Modern Language Association (MLA)
Akanda, Md Rashedunnabi…[et al.]. In Vivo and In Vitro Hepatoprotective Effects of Geranium koreanum Methanolic Extract via Downregulation of MAPKCaspase-3 Pathway. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1154666
American Medical Association (AMA)
Akanda, Md Rashedunnabi& Kim, In-Shik& Ahn, Dongchoon& Tae, Hyun-Jin& Tian, Weishun& Nam, Hyeon Hwa…[et al.]. In Vivo and In Vitro Hepatoprotective Effects of Geranium koreanum Methanolic Extract via Downregulation of MAPKCaspase-3 Pathway. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1154666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154666