Identifying Synergistic Mechanisms of Multiple Ingredients in Shuangbai Tablets against Proteinuria by Virtual Screening and a Network Pharmacology Approach
Joint Authors
Yu, Meixiang
Yang, Wanhua
Ma, Xiaoqin
Hao, Chenxia
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-07
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Shuangbai Tablets (SBT), a traditional herbal mixture, has shown substantial clinical efficacy.
However, a systematic mechanism of its active ingredients and pharmacological mechanisms of action against proteinuria continues being lacking.
A network pharmacology approach was effectual in discovering the relationship of multiple ingredients and targets of the herbal mixture.
This study aimed to identify key targets, major active ingredients, and pathways of SBT against proteinuria by network pharmacology approach combined with thin layer chromatography (TLC).
Human phenotype (HP) disease analysis, gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and molecular docking were used in this study.
To this end, a total of 48 candidate targets of 118 active ingredients of SBT were identified.
Network analysis showed PTGS2, ESR1, and NOS2 to be the three key targets, and beta-sitosterol, quercetin, and berberine were the three major active ingredients; among them one of the major active ingredients, quercetin, was discriminated by TLC.
These results of the functional enrichment analysis indicated that the most relevant disease including these 48 candidate proteins is proteinuria, SBT treated proteinuria by sympathetically regulating multiple biological pathways, such as the HIF-1, RAS, AGE-RAGE, and VEGF signaling pathways.
Additionally, molecular docking validation suggested that major active ingredients of SBT were capable of binding to HIF-1A and VEGFA of the main pathways.
Consequently, key targets, major active ingredients, and pathways based on data analysis of SBT against proteinuria were systematically identified confirming its utility and providing a new drug against proteinuria.
American Psychological Association (APA)
Ma, Xiaoqin& Yu, Meixiang& Hao, Chenxia& Yang, Wanhua. 2020. Identifying Synergistic Mechanisms of Multiple Ingredients in Shuangbai Tablets against Proteinuria by Virtual Screening and a Network Pharmacology Approach. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1154717
Modern Language Association (MLA)
Ma, Xiaoqin…[et al.]. Identifying Synergistic Mechanisms of Multiple Ingredients in Shuangbai Tablets against Proteinuria by Virtual Screening and a Network Pharmacology Approach. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1154717
American Medical Association (AMA)
Ma, Xiaoqin& Yu, Meixiang& Hao, Chenxia& Yang, Wanhua. Identifying Synergistic Mechanisms of Multiple Ingredients in Shuangbai Tablets against Proteinuria by Virtual Screening and a Network Pharmacology Approach. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1154717
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154717