Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies
Joint Authors
Yin, Zhongqiong
Huang, Xiang-Yue
Fu, Hua-Lin
Tang, Hua-Qiao
Zhang, Wei
Shu, Gang
Yin, Li-Zi
Zhao, Ling
Yan, Xiao-Rong
Lin, Ju-Chun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The objectives of this study were to develop and optimize ultrasound-assisted extraction (UAE) for shikonin from Arnebia euchroma using response surface methodology (RSM) and to evaluate the antimicrobial activity of shikonin.
The maximum yield of shikonin was 1.26% under the optimal extraction conditions (ultrasound power, 93 W; time, 87 min; temperature, 39°C; and liquid-solid ratio, 11 : 1).
Shikonin showed inhibitory activity against standard strains and clinical isolates to varying extents (MICs ranging from 128 to 1024 μg/mL, MBCs ranging from 256 to 2048 μg/mL), and it was more effective for Gram-positive bacteria as indicated by lower MIC and MBC values.
Time-kill curves revealed that antibacterial activity of shikonin exhibited a dose-response relationship.
In summary, via this study, we identified ultrasound-assisted RSM as the optimal extraction method for shikonin, which is a potential material for the treatment of bacterial infections.
American Psychological Association (APA)
Huang, Xiang-Yue& Fu, Hua-Lin& Tang, Hua-Qiao& Yin, Zhongqiong& Zhang, Wei& Shu, Gang…[et al.]. 2020. Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1154739
Modern Language Association (MLA)
Huang, Xiang-Yue…[et al.]. Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1154739
American Medical Association (AMA)
Huang, Xiang-Yue& Fu, Hua-Lin& Tang, Hua-Qiao& Yin, Zhongqiong& Zhang, Wei& Shu, Gang…[et al.]. Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1154739
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154739