Huayu Wan Prevents Lewis Lung Cancer Metastasis in Mice via the Platelet Pathway

Joint Authors

Yu, Ming-Wei
Wang, Xiao-Min
Sun, Xu
Ma, Yunfei
Li, Guangda
Cao, Kexin
Yang, Guowang

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-07-27

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Medicine

Abstract EN

Objective.

To study the mechanism of Huayu Wan on the metastasis of Lewis lung cancer in mice via the platelet pathway.

Method.

Construction of the lung metastasis model by injection of Lewis cells through the tail vein.

The next day, 72 mice were randomly divided into the Huayu Wan group (HYW), the aspirin group, the control group, and the normal group .

Treatment was given for 5 days per week for a total of 16 days.

The size and distribution of lung metastases were observed.

Thromboelastography was used to detect platelet function, flow cytometry was used to analyze platelet activation, and ELISA was used to detect platelet tumor metastasis-related factor expression.

Result.

Lung weight in the control group was significantly higher than that in the HYW group P<0.05.

The distribution of lung metastases in the control group was obviously more than that in the HYW group.

The thromboelastogram showed that the R value of the control group was significantly lower than the normal group, while the R values of the HYW and aspirin groups were higher than the control group P<0.05.

Flow cytometry analysis showed that the expression of CD62P in platelet-rich plasma in the control group was significantly higher than that in the normal group, while the expression of CD62P in the HYW and aspirin groups was lower than that in the control group P<0.05.

In addition, ELISA showed that the expression of VEGF, bFGF, and CD62P in serum of the HYW group was significantly decreased than the control group P<0.05, and the expression of VEGF and bFGF in serum of the aspirin group was significantly decreased than the control group P<0.05.

Conclusion.

The mechanism of Huayu Wan inhibiting the metastasis of lung cancer in mice may be related to the improvement of blood hypercoagulability, the inhibition of platelet activation, and the expression of VEGF, bFGF, and CD62P.

American Psychological Association (APA)

Ma, Yunfei& Li, Guangda& Sun, Xu& Cao, Kexin& Wang, Xiao-Min& Yang, Guowang…[et al.]. 2020. Huayu Wan Prevents Lewis Lung Cancer Metastasis in Mice via the Platelet Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1154764

Modern Language Association (MLA)

Ma, Yunfei…[et al.]. Huayu Wan Prevents Lewis Lung Cancer Metastasis in Mice via the Platelet Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1154764

American Medical Association (AMA)

Ma, Yunfei& Li, Guangda& Sun, Xu& Cao, Kexin& Wang, Xiao-Min& Yang, Guowang…[et al.]. Huayu Wan Prevents Lewis Lung Cancer Metastasis in Mice via the Platelet Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1154764

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1154764