Antineuroinflammatory Effects of Modified Wu-Zi-Yan-Zong Prescription in β-Amyloid-Stimulated BV2 Microglia via the NF-κB and ERKp38 MAPK Signaling Pathways
Joint Authors
Jiang, Yong
Yu, Qian
Song, Fang-Jiao
Chen, Jin-Feng
Dong, Xin
Zeng, Ke-Wu
Tu, Peng-Fei
Wang, Xue-Mei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Modified Wu-Zi-Yan-Zong prescription (MWP), a traditional Chinese medicinal decoction, has possessed the neuroprotective and anti-inflammatory properties.
The mechanisms associated with these properties, however, are not completely understood.
We designed the experiments to elucidate the antineuroinflammatory property of MWP in BV2 microglia activated by β-amyloid (Aβ), which is a characteristic feature of Alzheimer’s disease (AD).
The composition of MWP was studied using HPLC.
BV2 microglia cells were then treated with Aβ in the presence or absence of MWP.
The effects of MWP treatment on Aβ-activated neuroinflammation were determined using PCR, western blotting, and immunofluorescence staining.
MWP significantly inhibited the mRNA expression of inflammatory mediators such as IL-1β, IL-6, TNF-α, and MCP-1, as well as the expression of inducible nitric oxide synthase (iNOS) in Aβ-activated BV2 microglia.
MWP also inhibited the nuclear translocation and signaling pathway of nuclear factor kappa B (NF-κB) by suppressing inhibitor of nuclear factor-κB (IκB) degradation and downregulating IκB kinase β (IKKβ) phosphorylation.
Moreover, MWP decreased extracellular regulated protein kinase (ERK)/p38 mitogen-activated protein kinase (MAPK) phosphorylation, which is an important signaling pathway for proinflammatory gene expression.
We concluded that MWP could suppress neuroinflammatory responses in Aβ-activated BV2 microglia via the NF-κB and ERK/p38 MAPK signaling cascades and could prove an effective therapeutic agent for the prevention and treatment of neuroinflammatory diseases such as AD.
American Psychological Association (APA)
Yu, Qian& Song, Fang-Jiao& Chen, Jin-Feng& Dong, Xin& Jiang, Yong& Zeng, Ke-Wu…[et al.]. 2017. Antineuroinflammatory Effects of Modified Wu-Zi-Yan-Zong Prescription in β-Amyloid-Stimulated BV2 Microglia via the NF-κB and ERKp38 MAPK Signaling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154773
Modern Language Association (MLA)
Yu, Qian…[et al.]. Antineuroinflammatory Effects of Modified Wu-Zi-Yan-Zong Prescription in β-Amyloid-Stimulated BV2 Microglia via the NF-κB and ERKp38 MAPK Signaling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154773
American Medical Association (AMA)
Yu, Qian& Song, Fang-Jiao& Chen, Jin-Feng& Dong, Xin& Jiang, Yong& Zeng, Ke-Wu…[et al.]. Antineuroinflammatory Effects of Modified Wu-Zi-Yan-Zong Prescription in β-Amyloid-Stimulated BV2 Microglia via the NF-κB and ERKp38 MAPK Signaling Pathways. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154773
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154773