Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses
Joint Authors
Wang, Zheng-Tao
Ye, Zi
Dai, Jia-Rong
Zhang, Cheng-Gang
Lu, Ye
Wu, Lei-Lei
Gong, Amy G. W.
Xu, Hong
Tsim, Karl Wah-Keung
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM).
Because of the limited supply, D.
officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market.
The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species.
Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D.
officinale and D.
devonianum by comparing the chromatograms according to the characteristic peaks.
A HPLC coupled with electrospray ionization multistage mass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1 lignan in D.
officinale.
Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D.
officinale, and violanthin and isoviolanthin were identified to be specific for D.
officinale compared with D.
devonianum.
Then, two representative components were used as chemical markers.
A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied in distinguishing D.
officinale from D.
devonianum.
The results of this work have demonstrated that these developed analytical methods can be used to discriminate D.
officinale and D.
devonianum effectively and conveniently.
American Psychological Association (APA)
Ye, Zi& Dai, Jia-Rong& Zhang, Cheng-Gang& Lu, Ye& Wu, Lei-Lei& Gong, Amy G. W.…[et al.]. 2017. Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154820
Modern Language Association (MLA)
Ye, Zi…[et al.]. Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1154820
American Medical Association (AMA)
Ye, Zi& Dai, Jia-Rong& Zhang, Cheng-Gang& Lu, Ye& Wu, Lei-Lei& Gong, Amy G. W.…[et al.]. Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154820