Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate-Induced Constipation in Mice

Joint Authors

Xu, Hua-li
Sui, Da-yuan
Liu, Shan
Fu, Wenwen
Yu, Xiaofeng
Li, Yuangeng
Wu, Xueji
Hou, Yiping
Guo, Minyu

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-02-22

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Medicine

Abstract EN

Constipation is characterized by reduced number of bowel movements, dry stools, and difficult defecation.

Yangyin Tongmi capsule (YTC), a traditional Chinese formula, is used in the treatment of constipation, while the underlying mechanisms remain unknown.

Herein, this work attempted to prove the effects of YTC on constipation treatment and its possible mechanisms.

KM mice were randomly divided into four groups (n = 10/group) and treated with double distilled water (Control), diphenoxylate (Model: 10 mg/kg), or diphenoxylate plus low-dose YTC (L-YTC: 0.6 g/kg) or high-dose YTC (H-YTC: 1.2 g/kg).

The data indicated that YTC can significantly shorten the discharge time of the first black stool, improve intestinal propulsion rate, and increase the water content and quantity of feces in mice.

ELISA suggested that YTC regulate the content of intestinal hormones and neurotransmitters, such as motilin (MTL), gastrin (GT), somatostatin (SST), substance P (SP), acetylcholine (Ach), and nitric oxide (NO).

The expression levels of aquaporin 3 (AQP3) and aquaporin 8 (AQP8) in the colon were examined by immunohistochemistry.

In the meantime, the expression levels of P2X2, C-kit, and stem cell factor (SCF) in the colon were examined by western blot analysis.

The results of this study suggest that YTC has mitigative effects on diphenoxylate-induced constipation by regulating the content of intestinal hormones and neurotransmitters and regulating the expression of related proteins in the colon.

American Psychological Association (APA)

Liu, Shan& Sui, Da-yuan& Fu, Wenwen& Yu, Xiaofeng& Li, Yuangeng& Wu, Xueji…[et al.]. 2020. Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate-Induced Constipation in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1154843

Modern Language Association (MLA)

Liu, Shan…[et al.]. Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate-Induced Constipation in Mice. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1154843

American Medical Association (AMA)

Liu, Shan& Sui, Da-yuan& Fu, Wenwen& Yu, Xiaofeng& Li, Yuangeng& Wu, Xueji…[et al.]. Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate-Induced Constipation in Mice. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1154843

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1154843