Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy
Joint Authors
Guamán Ortiz, Luis Miguel
Romero-Benavides, Juan C.
Suarez, Alirica I.
Torres-Aguilar, Stephania
Castillo-Veintimilla, Paola
Samaniego-Romero, Jimmy
Ortiz-Diaz, Kevin
Bailon-Moscoso, Natalia
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-01
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Traditional herbal medicine has become an important alternative in the treatment of various cancer types, including colon cancer, which represents one of the main health problems around the world.
Therefore, the search for new therapies to counteract this disease is very active.
Grias neuberthii is an endemic plant located in the Ecuadorian Amazon region, which has been used in traditional medicine for its pharmacological properties, including its ability to inhibit tumor cell growth, although scientific studies are limited.
We have analyzed the effect of this plant on two colon carcinoma cell lines, that is, RKO (normal p53) and SW613-B3 (mutated p53) cells.
Among several extracts obtained from various parts of G.
neuberthii plant, we identified the extract with the greatest cytotoxic potential, derived from the stem bark.
The cytotoxic effect was similar on both cell lines, thus indicating that it is independent of the status of p53.
However, significant differences were observed after the analysis of colony formation, with RKO cells being more sensitive than SW613-B3.
No evidence for apoptotic markers was recorded; nevertheless, both cell lines showed signs of autophagy after the treatment, including increased Beclin-1 and LC3-II and decreased p62.
Finally, three chemical compounds, possibly responsible for the effect observed in both cell lines, were identified: lupeol (1), 3′-O-methyl ellagic acid 4-O-β-D-rhamnopyranoside (2), and 19-α-hydroxy-asiatic acid monoglucoside (3).
American Psychological Association (APA)
Guamán Ortiz, Luis Miguel& Romero-Benavides, Juan C.& Suarez, Alirica I.& Torres-Aguilar, Stephania& Castillo-Veintimilla, Paola& Samaniego-Romero, Jimmy…[et al.]. 2020. Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1154875
Modern Language Association (MLA)
Guamán Ortiz, Luis Miguel…[et al.]. Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1154875
American Medical Association (AMA)
Guamán Ortiz, Luis Miguel& Romero-Benavides, Juan C.& Suarez, Alirica I.& Torres-Aguilar, Stephania& Castillo-Veintimilla, Paola& Samaniego-Romero, Jimmy…[et al.]. Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1154875
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154875