Effects of Qutan Huoxue Formula on the SOCS1TLR4 Signaling Pathway in NASH Model Mice
Joint Authors
Yin, Yue
Peng, Mengyun
Zheng, Ding
Zhang, Yurong
Wang, Jing
Zhu, Xiaoning
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The purpose of this study was to investigate the effects of Qutan Huoxue Formula (QHF) on liver injury in mice with nonalcoholic steatohepatitis (NASH) by upregulating SOCS1 to inhibit the TLR4/NF-κB signaling pathway.
Thirty male C57BL/6J mice (20–22 g) were randomly divided into the normal diet group (ND group), methionine- and choline-deficient diet group (MCD group), and Qutan Huoxue Formula group (QHF group).
Mice in the ND group were fed a regular diet, while mice in other two groups were fed MCD diet.
After successful molding, the QHF group was gavaged by QHF.
The ND group and MCD group were gavaged by the same volume of normal saline, once a day.
During the period of gavaging, all mice continue to be fed MCD fodder except for the ND group.
All mice were killed at 8 w.
H&E staining and Oil Red O staining were used to observe the pathological changes of liver tissues.
Serum level of ALT, AST, TC, and TG was detected by enzyme-linked immunosorbent assay.
The expression of liver SOCS1, TLR4, Myd88, and NF-κB was detected by real-time PCR, immunohistochemistry, and Western blot.
QHF can significantly reduce the serum levels of ALT, AST, TC, and TG of NASH mice and reduce the degree of liver fat degeneration and inflammation.
It also can decrease both mRNA and protein expressions of liver TLR4, Myd88, and NF-κB.
The mRNA expression of SOCS1 increased, while the SOCS1 protein expression decreased.
In conclusion, QHF can significantly alleviate hepatic steatosis and inflammation in NASH mice by upregulating SOCS1 to inhibit the TLR4/NF-κB signaling pathway.
American Psychological Association (APA)
Zhang, Yurong& Zhu, Xiaoning& Zheng, Ding& Yin, Yue& Peng, Mengyun& Wang, Jing. 2020. Effects of Qutan Huoxue Formula on the SOCS1TLR4 Signaling Pathway in NASH Model Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1154877
Modern Language Association (MLA)
Zhang, Yurong…[et al.]. Effects of Qutan Huoxue Formula on the SOCS1TLR4 Signaling Pathway in NASH Model Mice. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1154877
American Medical Association (AMA)
Zhang, Yurong& Zhu, Xiaoning& Zheng, Ding& Yin, Yue& Peng, Mengyun& Wang, Jing. Effects of Qutan Huoxue Formula on the SOCS1TLR4 Signaling Pathway in NASH Model Mice. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1154877
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154877