Analysis of the Molecular Mechanism of Acute Coronary Syndrome Based on circRNA-miRNA Network Regulation
Joint Authors
Wang, Jie
Lin, Fei
Zhao, Guoan
Yang, YaMing
Guo, Quan
Xie, Mingzhang
Sun, Siyu
Wang, Xiulong
Li, Dongxu
Zhang, Guhao
Li, Meng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-29
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Background.
With the development of biological technology, biomarkers for the prevention and diagnosis of acute coronary syndrome (ACS) have become increasingly evident.
However, the study of novel circular RNAs (circRNAs) in ACS is still in progress.
This study aimed to investigate whether the regulation of circRNA-miRNA networks is involved in ACS pathogenesis.
Methods.
We used microarray analysis to detect significantly expressed circRNAs and miRNAs in the peripheral blood of patients in the control group (CG) and ACS groups, including an unstable angina pectoris (UAP) group and an acute myocardial infarction (AMI) group.
A circRNA-miRNA interaction network analysis was carried out with open-source bioinformatics.
The gene ontology (GO), pathway, and disease enrichment analyses for differentially expressed circRNAs were further analysed with hierarchical clustering.
Results.
A total of 266 circRNAs (121 upregulated and 145 downregulated, P<0.05, fold change FC ≥2) and 3 miRNAs (1 upregulated and 2 downregulated, P<0.05, FC ≥ 1.2) were differentially expressed in the ACS groups compared with those in the CG.
In addition, among these expressed circRNAs and miRNAs, a single circRNA could bind to more than 1–100 miRNAs, and vice versa.
Next, an AMI-UAP network, an AMI-CG network, a UAP-CG network, and an AMI-CG-UAP network were constructed.
The top 30 enriched GO terms among the three groups were emphasized as differentially expressed.
Disease enrichment analysis showed that these differentially expressed circRNAs are involved in the pathogenesis of cardiovascular diseases.
KEGG pathway analysis was performed to identify pathways associated with circRNAs targeting mRNAs.
Conclusion.
CircRNAs are closely related to the pathological process of ACS via a mechanism that may be related to the up- or down-regulation of circRNAs and miRNAs and circRNA-miRNA coexpression.
The metabolic pathways, signalling pathways, and diseases affected by these circRNAs can be predicted by enrichment analysis.
American Psychological Association (APA)
Lin, Fei& Yang, YaMing& Guo, Quan& Xie, Mingzhang& Sun, Siyu& Wang, Xiulong…[et al.]. 2020. Analysis of the Molecular Mechanism of Acute Coronary Syndrome Based on circRNA-miRNA Network Regulation. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1154889
Modern Language Association (MLA)
Lin, Fei…[et al.]. Analysis of the Molecular Mechanism of Acute Coronary Syndrome Based on circRNA-miRNA Network Regulation. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1154889
American Medical Association (AMA)
Lin, Fei& Yang, YaMing& Guo, Quan& Xie, Mingzhang& Sun, Siyu& Wang, Xiulong…[et al.]. Analysis of the Molecular Mechanism of Acute Coronary Syndrome Based on circRNA-miRNA Network Regulation. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1154889
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154889