Evaluation of Methanol-Dichloromethane Extract of Stemonocoleus micranthus Harms (Fabaceae) Stem Bark for Anti-Inflammatory and Immunomodulatory Activities
Joint Authors
Mbaoji, Florence Nwakaego
Onwuka, Akachukwu Marytheresa
Onu, Sunday
Peter, Ikechukwu Emmanuel
Nweze, Justus Amuche
Okonta, Lilian Eleje
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
The stem bark decoction of Stemonocoleus micranthus Harms (Fabaceae) is most widely used traditionally as a remedy for various diseases such as malaria and boil.
In this study, the anti-inflammatory and immunomodulatory activities of the methanol-dichloromethane extract (MDE) from the stem bark of the plant in rodents were evaluated.
Methods.
The carrageenan-induced rat paw oedema, cotton pellet-induced granuloma in rat, and xylene-induced ear oedema in mice were used to study the anti-inflammatory activity of methanol-dichloromethane extract of Stemonocoleus micranthus (MDESm) (100, 200, and 400 mg/kg).
The effects of MDESm (100, 200, and 400 mg/kg) on cyclophosphamide-induced immunosuppression, neutrophil adhesion, carbon clearance, and haematological and biochemical parameters were carried out to study its immunomodulatory activity in mice.
Result.
MDESm (100 mg/kg, p.o.) significantly (p<0.05) inhibited carrageenan-induced oedema by 57.1% at 5th h posttreatment compared with control.
At 100 mg/kg, p.o., MDESm significantly (p<0.05) reduced cotton pellet-induced granuloma by 39.28% and nonsignificantly reduced xylene-induced ear oedema by 34.1%.
Treatment with MDESm (100 and 400 mg/kg) nonsignificantly abolished the neutropenia caused by cyclophosphamide with a percentage neutrophil reduction of 0 and −14.86%, respectively, while MDESm (200 mg/kg) and levamisole (50 mg/kg) had a nonsignificant reduction in neutrophil count (10.16 and 31.40%), respectively, all compared to the distilled water-treated group with a neutrophil count of −9.82%.
MDESm at doses of 100 and 200 mg/kg increased phagocytic index by 0.0447 ± 0.00762 and 0.0466 ± 0.00703, respectively, although not significantly when compared to the control group with a value of 0.0226 ± 0.02117.
There was a decrease in WBC and lymphocyte counts in MDESm- (200 mg/kg) treated group, suggesting immunosuppressive potential at this dose.
MDESm caused a dose-dependent decrease in ALT and core liver enzymes, suggesting a hepatoprotective effect.
The acute toxicity test revealed that MDESm is safe in mice with an oral lethal dose (LD50) of >5 g/kg.
Conclusion.
The methanol-dichloromethane extract of Stemonocoleus micranthus Harms possesses mild anti-inflammatory and immunomodulatory activities which may be more pronounced upon fractionation and purification.
Therefore, more investigations are needed to explore these activities further.
American Psychological Association (APA)
Mbaoji, Florence Nwakaego& Onwuka, Akachukwu Marytheresa& Onu, Sunday& Peter, Ikechukwu Emmanuel& Nweze, Justus Amuche& Okonta, Lilian Eleje. 2020. Evaluation of Methanol-Dichloromethane Extract of Stemonocoleus micranthus Harms (Fabaceae) Stem Bark for Anti-Inflammatory and Immunomodulatory Activities. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1154938
Modern Language Association (MLA)
Mbaoji, Florence Nwakaego…[et al.]. Evaluation of Methanol-Dichloromethane Extract of Stemonocoleus micranthus Harms (Fabaceae) Stem Bark for Anti-Inflammatory and Immunomodulatory Activities. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1154938
American Medical Association (AMA)
Mbaoji, Florence Nwakaego& Onwuka, Akachukwu Marytheresa& Onu, Sunday& Peter, Ikechukwu Emmanuel& Nweze, Justus Amuche& Okonta, Lilian Eleje. Evaluation of Methanol-Dichloromethane Extract of Stemonocoleus micranthus Harms (Fabaceae) Stem Bark for Anti-Inflammatory and Immunomodulatory Activities. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1154938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154938